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Electrochemical DNA-Based Sensor for Organophosphorus Pesticides Detection

  • Giulia Selvolini
  • Ioana Băjan
  • Oana Hosu
  • Cecilia Cristea
  • Robert Săndulescu
  • Giovanna MarrazzaEmail author
Conference paper
Part of the Lecture Notes in Electrical Engineering book series (LNEE, volume 539)

Abstract

In this work, we propose an electrochemical DNA-based sensor for sensitive detection of organophosphorus pesticides. To improve the sensitivity of the DNA-based sensor, polyaniline film and gold nanoparticles were progressively electrodeposited on the graphite screen-printed electrode surface by cyclic voltammetry. Gold nanoparticles were then employed as platform for the immobilization of thiol-tethered DNA oligonucleotide sequence complementary to the selected biotinylated DNA aptamer for profenofos detection. Streptavidin-alkaline phosphatase enzyme conjugate was then added to trace the affinity reaction through the hydrolysis of 1-naphthyl phosphate to 1-naphthol, which was then detected by differential pulse voltammetry. A decrease of the signal was obtained when the pesticide concentration was increased, making the sensor work as signal off sensor.

Keywords

Pesticide Aptamer DNA Competitive assay Biosensor 

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Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.University of FlorenceSesto FiorentinoItaly
  2. 2.University of Medicine and Pharmacy “Iuliu Hatieganu”Cluj NapocaRomania

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