Bioactive Profile of Edible Ripened Split Beans of Three Wild Landraces of Coastal Canavalia

  • Suvarna J. Shreelalitha
  • Prabhavathi Supriya
  • Kandikere R. Sridhar


The coastal dwellers of Southwest India consume ripened split beans of wild legumes Canavalia cathartica and C. maritima as nutritional source. Removal of seed coat and testa of ripened beans followed by pressure-cooking eliminates antinutritional factors substantially without impinging antioxidant or health-promoting potential. This study compares bioactive potential of uncooked and pressure-cooked ripened split beans of three landraces of Canavalia of southwest coast of India. Contents of total phenolics, tannins, and orthodihydric phenols in ripened split beans of all landraces were substantially lowered on cooking. Uncooked and cooked ripened split beans were devoid of trypsin inhibition activity, while hemagglutinin activity was completely eliminated in all landraces except for O+ blood group in C. maritima of coastal sand dune (decreased to one-third). Pressure-cooking decreased the bioactive components to ideal concentration to serve as nutraceuticals and antioxidants, which has potential implications on human health. Overall, the ripened split beans of coastal landraces of wild Canavalia are a rich nutritional source with potential bioactive components which serve as future nutritional health food commodity and warrant further insight on domestication for sustainable exploitation.


Wild legumes Mangroves Coastal sand dunes Ethnic food Nutraceutical Vitamin C Antioxidant activity Reducing power 



Authors are grateful to Mangalore University for permission to carry out this study in the Department of Biosciences. Sridhar K.R. gratefully acknowledges the UGC-BSR Faculty Fellowship by the UGC, New Delhi. Shreelalitha S.J. acknowledges the UGC, New Delhi, for the award of research fellowship under the scheme RFSMS. Supriya P. is grateful to BRNS, Bhabha Atomic Research Centre, Mumbai, for the award of research fellowship.


  1. Arora RK, Chandel KPS, Joshi BS, Pant KC (1980) Rice bean: tribal pulse of eastern India. Econ Bot 34:260–263CrossRefGoogle Scholar
  2. Arun AB, Beena KR, Raviraja NS, Sridhar KR (1999) Coastal sand dunes - a neglected ecosystem. Curr Sci 77:19–21Google Scholar
  3. Arun AB, Sridhar KR, Raviraja NS, Schmidt E, Jung K (2003) Nutritional and antinutritional components of Canavalia spp. seeds from the west coast sand dunes of India. Plant Foods Hum Nutr 58:1–13CrossRefGoogle Scholar
  4. Bhagya B, Sridhar KR (2009) Ethnobiology of coastal sand dune legumes of southwest India. Indian J Tradit Knowl 9:611–620Google Scholar
  5. Bhagya B, Sridhar KR, Seena S, Young C-C, Arun AB, Nagaraja KV (2006) Nutritional qualities and in vitro starch digestibility of ripened Canavalia cathartica beans of coastal sand dunes of southern India. Elec J Environ Agric Food Chem 5:1241–1252Google Scholar
  6. Bhagya B, Sridhar KR, Seena S, Bhat R (2007) Nutritional qualities of ripened beans of mangrove legume Canavalia cathartica Thouars. J Agric Technol 3:255–274Google Scholar
  7. Bhagya B, Sridhar KR, Raviraja NS, Young C-C, Arun AB (2009) Nutritional and biological qualities of ripened beans of Canavalia maritima of coastal sand dunes of India. C R Biol 332:25–33CrossRefGoogle Scholar
  8. Bhat R, Karim AA (2009) Exploring the nutritional potential of wild and underutilized legumes. Compr Rev Food Sci Food Saf 8:305–331CrossRefGoogle Scholar
  9. Boye J, Zare F, Pletch P (2010) Pulse proteins: Processing, characterization, functional properties and applications in food and feed. Food Res Int 43:414–431CrossRefGoogle Scholar
  10. Brown DL (2005) Canavanine-induced longevity in mice may require diets with greater than 15.7% protein. Nutr Metab 2:7. Scholar
  11. Burns R (1971) Methods for estimation of tannins in grain sorghum. Agron J 63:511–512CrossRefGoogle Scholar
  12. Cardador-Martinez A, Loarca-Pina G, Oomah BD (2002) Antioxidant activity in common beans (Phaseolus vulgaris L.). J Agric Food Chem 50:6975–6980CrossRefGoogle Scholar
  13. Combs GF, Gray WP (1998) Chemopreventive agents: selenium. Pharmacol Ther 79:179–192CrossRefGoogle Scholar
  14. D’Cunha M, Sridhar KR (2010) L-canavanine and L-arginine in two wild legumes of the genus Canavalia. Inst Integr Omics Appl Biotechnol J 1:29–33Google Scholar
  15. Enneking D, Wink M (2000) Towards the elimination of anti-nutritional factors in grain legumes. In: Knight R (ed) Current plant science and biotechnology in agriculture, vol 34. Kluwer Academic Publishers, Dordrecht, pp 375–384Google Scholar
  16. Fearon WR, Bell EA (1954) Canavanine: detection and occurrence in Colutea arborescens. J Biochem 59:221–224CrossRefGoogle Scholar
  17. Gunjatkar N, Vartak VD (1982) Enumeration of wild legumes from Pune District, Maharashtra. J Econ Taxon Bot 3:1–9Google Scholar
  18. Kakade ML, Rackis JJ, McGhee JE, Puski G (1974) Determination of trypsin inhibitor activity of soy products, a collaborative analysis of an improved procedure. Cereal Chem 51:376–382Google Scholar
  19. Koratkar R, Rao AV (1997) Effect of soybean saponins on azoxymethane-induced preneoplastic lesions in the colon of mice. Nutr Cancer 27:206–209CrossRefGoogle Scholar
  20. Liener I (1980) Heat-labile antinutritional factors. In: Summerfield J, Bunting AH (eds) Advances in legume science. Royal Botanic Gardens, Kew, pp 151–170Google Scholar
  21. Mahadevan A, Sridhar R (1985) Methods in physiological plant pathology, 3rd edn. Sivakami Publications, ChennaiGoogle Scholar
  22. McDougall GJ, Stewart D (2005) The inhibitory effects of berry polyphenols on digestive enzymes. Biofactors 23:189–195CrossRefGoogle Scholar
  23. Mohan VR, Janardhanan K (1995) Chemical determination of nutritional and antinutritional properties in tribal pulses. J Food Sci Technol 32:465–469Google Scholar
  24. Nakanishi H (1988) Dispersal ecology of the maritime plants in the Ryukyu Islands, Japan. Ecol Res 3:163–174CrossRefGoogle Scholar
  25. Narayanan MKR, Kumar NA (2007) Generated knowledge and changing trends in utilization of wild edible greens in Western Ghats. Indian J Tradit Knowl 6:204–216Google Scholar
  26. Niveditha VR, Sridhar KR (2012) Concanavalin and canavanine in seeds of coastal sand dune legumes (Canavalia). Adv Biotech 11:30–34Google Scholar
  27. Occenã IV, Majica E-RE, Merca FE (2007) Isolation of partial characterization of a lectin from the seeds of Artocarpus camansi Blanco. Asian J Plant Sci 6:757–764CrossRefGoogle Scholar
  28. Oliveira AEA, Sales MP, Machado OLT, Fernandes KVS, Xavier-Filho J (1999) The toxicity of Jack bean (Canavalia ensiformis) cotyledon and seed coat proteins to the cowpea weevil (Callosobruchus maculatus). Entomol Exp Appl 92:249–255CrossRefGoogle Scholar
  29. Oyaizu M (1986) Studies on products of browning reactions: Antioxidative activities of products of browning reaction prepared from glucosamine. Jpn J Nutr 44:307–315CrossRefGoogle Scholar
  30. Prieto P, Pineda M, Aguilar M (1999) Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Anal Biochem 269:337–341CrossRefGoogle Scholar
  31. Rao TA, Sherieff AN (2002) Coastal Ecosystem of the Karnataka State, India II - Beaches. Karnataka Association for the Advancement of Science, BangaloreGoogle Scholar
  32. Rao TA, Suresh PV (2001) Coastal ecosystems of the Karnataka State, India I - Mangroves, Karnataka Association for the Advancement of Science, BangaloreGoogle Scholar
  33. Roe JH (1954) Chemical determination of ascorbic, dehydroascorbic and diketogluconic acids. In: Glick D (ed) Methods of biochemical analysis, vol 1. InterScience Publishers, New York, pp 115–139Google Scholar
  34. Rosset J, Bärlocher F, Oertli JJ (1982) Decomposition of conifer needles and deciduous leaves in two Black Forest and two Swiss Jura streams. Int Rev Gesamten Hydrobiol 67:695–711Google Scholar
  35. Seena S, Sridhar KR (2006) Nutritional and microbiological features of little known legumes, Canavalia cathartica Thouars and C. maritima Thouars of the southwest coast of India. Curr Sci 90:1638–1650Google Scholar
  36. Seena S, Sridhar KR, Arun AB (2007) Canavalia cathartica of southwest coast of India - a neglected wild legume. Plant Gen Res Newsl 150:16–20Google Scholar
  37. Shahidi F, Wanasundara PK (1992) Phenolic antioxidants. Crit Rev Food Sci Nutr 32:67–103CrossRefGoogle Scholar
  38. Shamsuddin AM, Vucenik I, Cole KE (1997) IP6: a novel anticancer agent. Life Sci 61:343–354CrossRefGoogle Scholar
  39. Singh RJ, Chung GH, Nelson RL (2007) Landmark research in legumes. Genome 50:525–537CrossRefGoogle Scholar
  40. Sridhar KR, Bhagya B (2007) Coastal sand dune vegetation: a potential source of food, fodder and pharmaceuticals. Livest Res Rural Dev 19:Article # 84: Scholar
  41. Sridhar KR, Seena S (2006) Nutritional and antinutritional significance of four unconventional legumes of the genus Canavalia - a comparative study. Food Chem 99:267–288CrossRefGoogle Scholar
  42. Sridhar KR, Shreelalitha SJ, Supriya P, Arun AB (2016) Nutraceutical attributes of ripened split beans of three Canavalia landraces. J Agric Technol 12:1275–1295Google Scholar
  43. StatSoft Inc. (2008) Statistica, Version # 8. StatSoft, Tulsa, Oklahoma, USAGoogle Scholar
  44. Supriya P, Sridhar KR, Ganesh S (2014) Fungal decontamination and enhancement of shelf life of edible split beans of wild legume Canavalia maritima by the electron beam irradiation. Radiat Phys Chem 96:5–11CrossRefGoogle Scholar
  45. Tapiero H, Tew KD, Nguyen BG, Mathe G (2002) Polyphenols: do they play a role in the prevention of human pathologies? Biomed Pharmacother 56:200–207CrossRefGoogle Scholar
  46. Thomas FA, Rosenthal GA, Gold DV, Dickey K (1986) Growth inhibition of a rat colon tumor by L-canavanine. Cancer Res 46:2898–2903PubMedGoogle Scholar
  47. Vadivel V, Janardhanan K (2005) Nutritional and antinutritional characteristics of seven South Indian wild legumes. Plant Foods Hum Nutr 60:69–75CrossRefGoogle Scholar
  48. Viswanathan MB, Thangadurai D, Tamilvendan K, Ramesh N (1999) Chemical analysis and nutritional assessment of Teramnus labialis (L.) Spreng. (Fabaceae). Plant Foods Hum Nutr 54:345–352CrossRefGoogle Scholar
  49. Viswanathan MB, Thangadurai D, Ramesh N (2001) Biochemical valuation of Neonotonia wightii (Wight and Arn.) Lackey (Fabaceae). Food Chem 75:275–279CrossRefGoogle Scholar
  50. Williams SE, Hunt GE (1967) Canavanine distribution in jackbean fruit during fruit growth. Planta 77:192–202PubMedGoogle Scholar

Copyright information

© Springer Nature Switzerland AG 2019

Authors and Affiliations

  • Suvarna J. Shreelalitha
    • 1
  • Prabhavathi Supriya
    • 2
  • Kandikere R. Sridhar
    • 2
  1. 1.Department of BiotechnologySt. Aloysius CollegeMangaloreIndia
  2. 2.Department of BiosciencesMangalore UniversityMangaloreIndia

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