Microwave-Accelerated Metal-Enhanced Fluorescence (MAMEF): A Rapid, < 10 Copy Number Detection Platform

  • Tonya M. Santaus
  • Chris D. GeddesEmail author
Part of the Reviews in Fluorescence book series (RFLU)


In this chapter, we review a three-piece assay called microwave-accelerated metal-enhanced fluorescence (MAMEF). This method is rapid and efficient for detecting various proteins and DNA/RNA fragments from a range of bacteria. Currently, bacterial detection and identification are underpinned by robust instrumentation and cell cultures. Molecular detection strategies are available to detect DNA/RNA and proteins in time periods that range from hours to days. These strategies use various reagents, costly instrumentation, and can be time-consuming.

The MAMEF assay eliminates the need to amplify DNA and culturing methods for detection and has been developed by our research group over several years for the detection of various bacteria. The assay is based on the principles of metal-enhanced fluorescence and has undergone extensive experimental testing and clinical validations. In this chapter, we subsequently review the overarching principles behind MAMEF, the development of protein assays for anthrax and myoglobin, and DNA hybridization assays for Salmonella, Chlamydia, and Gonorrhea. Microwave-accelerated metal-enhanced fluorescence offers the significant benefit of rapid protein and DNA/RNA detection without the need for thermo-cycling-based amplification and culturing methods, which are widely used today, despite their significant run times.


Microwave-accelerated metal-enhanced fluorescence DNA detection Protein detection Rapid detection Low-copy number detection 


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Copyright information

© Springer Nature Switzerland AG 2018

Authors and Affiliations

  1. 1.Institute of Fluorescence, Department of Chemistry and BiochemistryUniversity of Maryland, Baltimore CountyBaltimoreUSA
  2. 2.Institute of FluorescenceUniversity of Maryland Baltimore CountyBaltimoreUSA

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