GECIquant: Semi-automated Detection and Quantification of Astrocyte Intracellular Ca2+ Signals Monitored with GCaMP6f

  • Sharmila VenugopalEmail author
  • Rahul SrinivasanEmail author
  • Baljit S. KhakhEmail author
Part of the Springer Series in Computational Neuroscience book series (NEUROSCI)


Astrocytes display diverse and frequent intracellular Ca2+ fluctuations that are separable by virtue of their location within the cells, their magnitude, and their duration. Recently, the study of astrocyte Ca2+ signaling has rapidly advanced by the availability of genetically encoded Ca2+ indicators (GECIs) such as GCaMP3 and GCaMP6. The systematic use of GECIs is beginning to reveal the rules for astrocyte engagement within neural circuits in brain slices and in vivo. However, the richness and high numbers of Ca2+ signals that have been observed necessitate their routine detection within the complex morphology of astrocytes. To this end, in this chapter, we describe the development and features of GECIquant software that permits the semi-automated detection and quantification of astrocyte Ca2+ signals. Biological insights afforded by the use of GECIs and GECIquant are also described.


GECI GCaMP GECIquant Calcium Astrocytes Imaging Ca2+ microdomains Local waves Soma Territory 



The authors are supported by the NINDS (BSK, SV), NIMH (BSK), and CHDI Foundation (BSK) Awards.


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© Springer Nature Switzerland AG 2019

Authors and Affiliations

  1. 1.Department of PhysiologyDavid Geffen School of Medicine, University of California Los AngelesLos AngelesUSA
  2. 2.Department of NeurobiologyDavid Geffen School of MedicineLos AngelesUSA
  3. 3.Department of Integrative Biology & Physiology, Division of Life SciencesUniversity of California Los AngelesLos AngelesUSA
  4. 4.Department of Neuroscience and Experimental TherapeuticsTexas A&M University College of MedicineBryanUSA

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