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Hemagglutinin-Esterase Glycoprotein Gene of Bovine Coronavirus Delivered by Adenovirus Vector Induces Mucosal Immunity in Cotton Rats

  • M. E. Baca-Estrada
  • L. A. Babiuk
  • D. Yoo
Chapter
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 412)

Abstract

Adenoviruses replicate in the mucosal surfaces of the respiratory and intestinal tracts of humans and animals. Therefore, recombinant adenoviruses are attractive live vectors for antigen delivery to induce effective mucosal immune responses. Enteric coronavirus is an important cause of neonatal diarrhoea in calves resulting in significant economic losses due to the mortality and decreased productivity of the survivors. The hemagglutinin-esterase (HE) of bovine coronavirus (BCV) encodes a major viral membrane glycoprotein that elicits BCV-neutralizing antibodies (Deregt & Babiuk, 1987). We constructed a recombinant adenovirus vector to carry the HE gene of BCV in the early transcription region 3 of the adenovirus genome (Yoo et al. 1992). Testing of recombinant human adenovirus in animals has been difficult due to the highly restricted host range. We have used cotton rats as a model to test the immunogenicity of our recombinant adenovirus vector, since they have been shown to support replication of various adenovirus including human Ads (Pacini et al 1984).

References

  1. Deregt, D. and Babiuk, L. A. 1987. Monoclonal antibodies to bovine coronavirus: characteristics and topographical mapping of neutralizing epitopes on the E2 and E3 glycoproteins. Virology 161: 410.CrossRefPubMedGoogle Scholar
  2. Pacini, D. L., Dubovi, E. and Clyde, W. A. Jr. 1984 A new animal model for human respiratory tract disease due to adenovirus. J. Infec. Dis. 150: 92.CrossRefGoogle Scholar
  3. Yoo, D., Graham, L., Prevec, L., Parker, M. D., Benko, M., Zamb, T. and Babiuk, L. A. 1992 Synthesis and processing of the haemagglutinin-esterase glycoprotein of bovine coronavirus encoded in the E3 region of adenovirus. J. Gen. Virol. 73: 2591CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • M. E. Baca-Estrada
    • 1
  • L. A. Babiuk
    • 1
  • D. Yoo
    • 1
  1. 1.Veterinary Infectious Disease OrganizationUniversity of SaskatchewanSaskatoonCanada

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