A C-Terminal Sequencing Method Using Perfluoroacyl Anhydrides Vapor

  • K. Takamoto
  • M. Kamo
  • K. Satake
  • A. Tsugita

Abstract

The amino acid sequence of a protein of interest is usually one of the first pieces required in today’s molecular biology, be it for gene cloning or synthesis of immunoreactive peptides. To date, amino(N)-terminal sequencing using the Edman degradation procedure has almost exclusively provided such data. Methodologies for sequencing proteins from their carboxy(C)-termini have remained relatively primitive requiring much protein in return for little sequence information. Carboxypeptidase digestion is still the most widely used method despite its intrinsic limitations of substrate specificity and endoprotease contamination. Several chemical degradation methods have been reported (Stark, 1968; Yamashita,1971; Bailey et al., 1994), and a few automated C-terminal sequencers are almost available to the public.

Keywords

Fast Atom Bombardment Mass Spectrometry Fast Atom Bombardment Mass Spectrum Nitrobenzyl Alcohol Successive Degradation CNBr Cleavage 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • K. Takamoto
    • 1
  • M. Kamo
    • 1
  • K. Satake
    • 1
  • A. Tsugita
    • 1
  1. 1.Research Institute for Biosciences ScienceUniversity of TokyoYamazaki 2669, Noda 278Japan

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