Application: Aspergillus Oryzae as a Host for Production of Industrial Enzymes
Several groups have described the use of Aspergillus species, predominantly A. nidulans and A. niger, as expression hosts for synthesis of heterologous proteins, as reviewed recently by Gwynne (1992). At Novo Nordisk an expression system has been developed in A. oryzae (Christensen et al., 1988) and used for expression of several new industrial enzymes. Work in the Company has been focused on the production of secreted enzymes because purification of the product is simplified when the product can be isolated from the fermentation broth. Some of these enzymes are now available as commercial products, a situation which was made possible via high level expression in A. oryzae. In this paper some of the work carried out at Novo Nordisk in the development of A. oryzae into a host for production of industrial enzymes is described.
KeywordsLipase Production Aspergillus Nidulans Aspergillus Oryzae Industrial Enzyme Amylase Gene
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