Application: Aspergillus Oryzae as a Host for Production of Industrial Enzymes

  • Tove Christensen
Part of the Federation of European Microbiological Societies Symposium Series book series (FEMS, volume 69)

Abstract

Several groups have described the use of Aspergillus species, predominantly A. nidulans and A. niger, as expression hosts for synthesis of heterologous proteins, as reviewed recently by Gwynne (1992). At Novo Nordisk an expression system has been developed in A. oryzae (Christensen et al., 1988) and used for expression of several new industrial enzymes. Work in the Company has been focused on the production of secreted enzymes because purification of the product is simplified when the product can be isolated from the fermentation broth. Some of these enzymes are now available as commercial products, a situation which was made possible via high level expression in A. oryzae. In this paper some of the work carried out at Novo Nordisk in the development of A. oryzae into a host for production of industrial enzymes is described.

Keywords

Lipase Production Aspergillus Nidulans Aspergillus Oryzae Industrial Enzyme Amylase Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Barbesgaard, P., Heldt-Hansen, H.P. and Diderichsen B. (1992) On the safety of Aspergillus oryzae: A review. Appl. Microbiol. Biotechnol. 36, 569–572.PubMedCrossRefGoogle Scholar
  2. Cheevadhanarak, S., Saunders, G., Renno, D.V. and Flegel, T.W. (1991) Transformation of Aspergillus oryzae with a dominant selectable marker. J. Biotech. 19, 117–122.CrossRefGoogle Scholar
  3. Christensen, T., Woeldike, H., Boel, E., Mortensen, S.B., Hjortshoej, K., Thim L. and Hansen M.T. (1988) High level expression of recombinant genes in Aspergillus oryzae. Biotechnology 6, 1419–1422.CrossRefGoogle Scholar
  4. Corrick, C. M., Twomey A P. and Hynes M.J. (1987) The nucleotide sequence of the amdS gene of Aspergillus nidulans and the molecular characterization of 5’ mutations. Gene 53, 63–71.PubMedCrossRefGoogle Scholar
  5. Gomi, K., Iimura Y. and Hara S. (1987) Integrative transformation of Aspergillus oryzae with a Plasmid containing the Aspergillus nidulans argB gene. Agricol. Biol. Chem. 51, 2549–2555.CrossRefGoogle Scholar
  6. Gwynne, D.I., 1992, Foreign proteins, in “Applied Molecular Genetics of Filamentous Fungi” (Kinghorn, J.R. and Turner, G. Ed.), pp. 132–151. Blackie Academic & Professional, London.CrossRefGoogle Scholar
  7. Hynes, M.J., Corrick, C.M. and Littlejohn, K.J.M. and T.G. (1988) Identification of the sites of action for regulatory genes controlling the amdS gene of Aspergillus nidulans. Mol. Cell. Biol. 8, 2589–2596.PubMedGoogle Scholar
  8. Hynes, M.J., Corrick C.M. and King J.A. (1983) Isolation of genomic clones containing the amdS gene of Aspergillus nidulans and their use in the analysis of structural and regulatory mutations. Mol. Cell. Biol. 3, 1430–1439.PubMedGoogle Scholar
  9. Katz, M., Saleeba, J.A., Sapats, S.I. and Hynes M.J. (1990) A mutation affecting amdS expression in Aspergillus nidulans contains a triplication of a cis-acting regulatory sequence. Mol. Gen. Genet. 220, 373–376.PubMedCrossRefGoogle Scholar
  10. Mattern, I.E., Unkles, S., Kinghorn, J.R., Pouwels P.H. and van den Hondel, C.A.M.J.J. (1987) Transformation of Aspergillus oryzae using the A. niger pyrG gene. Mol. Gen. Genet. 210, 460–461.PubMedCrossRefGoogle Scholar
  11. May, G.S., J. Gambino, W. J.A. and N.R. Morris (1985) Identification and functional analysis of beta-tubulin genes by site specific integrative transformation in Aspergillus nidulans. J. Cell. Biol. 101, 712–719.PubMedCrossRefGoogle Scholar
  12. Unkles, S.E., Campbell, E.I., de Ruiter-Jacobs, Y.M.J.T., Brockhuijsen, M., Macro, J.A., Carrez, D., Contreras, R., van den Hondel, C.A.M.J.J. and Kinghom J.R. (1989) The development of a homologous transformation system for Aspergillus oryzae based on the nitrate assimilation pathway: A convenient and general selection system for filamentous fungal transformation. Mol. Gen. Genet. 218, 99–104.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • Tove Christensen
    • 1
  1. 1.Department of Fungal GeneticsNovo Nordisk A/SBagsvaerdDenmark

Personalised recommendations