The ARp1 Aspergillus Replicating Plasmid

  • John Clutterbuck
  • David Gems
  • Scott Robertson
Part of the Federation of European Microbiological Societies Symposium Series book series (FEMS, volume 69)

Abstract

Most plasmids used in the transformation of filamentous fungi depend for their maintenance on integration into the chromosome (Ballance 1991). Previous attempts to isolate plasmids capable of autonomous replication were unsuccessful. However, Johnstone (1985) had used a gene bank consisting of genomic inserts in the argB + plasmid pILJ16 (Figure 1) primarily to clone developmental genes, and reasoned that some members of this gene bank might contain inserts able to promote autonomous replication. Such a plasmid might replicate or be partitioned less efficiently than the chromosome and would therefore be identifiable by the instability of its transformants. On this basis three unstable transformants were selected and from one of these a plasmid was recovered which retransformed argB strains much more efficiently than the original vector. The properties of the Aspergillus replicating plasmid (ARp1) have been described by Gems et al (1991) and are summarised here.

Keywords

Inverted Repeat Transformation Frequency Gene Bank Aspergillus Nidulans Autonomous Replication 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bailance, D.J. (1991) Transformation systems for filamentous fungi, in “Molecular Industrial Mycology”, (Leong, S.A. and Berka, R.M., Eds) pp. 1–29. Marcel Dekker, New York.Google Scholar
  2. Brückner, B., Unkles, S.E., Weltring, K. and Kinghorn, J.R. (1992) Transformation of Gibberellafujikuroi: effect of the Aspergillus nidulans AMA1 sequence on frequency and integration. Curr. Genet. 22, 313–316.PubMedCrossRefGoogle Scholar
  3. Futcher, A.B. (1986) Copy number amplification of the 2μm circle plasmid of Saccharomyces cerevisiae. J. Theor. Biol. 119, 197–204.PubMedCrossRefGoogle Scholar
  4. Gems, D.H. and Clutterbuck, A.J. (1993) Cotransformation with autonomously replicating plasmids facilitates gene cloning from an Aspergillus nidulans gene library. Curr. Genet. (in press).Google Scholar
  5. Gems, D.H., Johnstone, I.L. and Clutterbuck, A.J. (1991) An autonomously replicating plasmid transforms Aspergillus nidulans at high frequency. Gene 98, 61–67.PubMedCrossRefGoogle Scholar
  6. Johnstone, I.L. (1985) Transformation of Aspergillus nidulans. Microbiol. Sci. 2, 307–311.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • John Clutterbuck
    • 1
  • David Gems
    • 1
  • Scott Robertson
    • 1
  1. 1.Department of GeneticsGlasgow UniversityGlasgowScotland, UK

Personalised recommendations