Cloning, Expression and Regulation of Angiotensin II Receptors

  • Tadashi Inagami
  • Yoshiaki Yamano
  • Smriti Bardhan
  • Shigeyuki Chaki
  • Deng-Fu Guo
  • Kenji Ohyama
  • Yoshikazu Kambayashi
  • Kyoko Takahashi
  • Toshihiro Ichiki
  • Satoshi Tsuzuki
  • Hua Tang
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 377)

Summary

Complementary DNAs for angiotensin II type 1 receptor isoforms AT1A and AT1B were cloned by expression cloning from bovine adrenal and rat vascular smooth muscles. Human AT, receptor was also cloned. Seven transmembrane structures emerged. The AT1 type receptor interacted with more than one type of G-proteins. The ligand binding site of AT1 involving Arg167, Lys199, and Asp263 has been identified by site directed mutagenesis. The regulation of the receptors occur at many stages. The isoform, AT2, was also expression cloned from rat pheochromocytoma cells. Although its ligand binding is not affected by stable GTP analogs, it is a seven transmembrane domain receptor. It mediates the modulations of phosphotyrosine phosphatase by angiotensin II and AT2 specific CGP42112A. The modulation was abolished by pertussis toxin. Thus, AT2 belongs to a new class of angiotensin receptors with unique signalling and regulatory mechanisms. AT1 mediates cellular growth. Interestingly, AT2 expression is inversely related to the mitogenic activity of cells.

Keywords

Vascular Smooth Muscle Cell Phosphotyrosine Phosphatase Bovine Adrenocortical Cell Transmembrane Domain Receptor Particulate Guanylate Cyclase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bottari SP, King IN, Reichlin S, Dahlstroem I, Lydon N and de Gasparo M: The angiotensin AT2 receptor stimulates protein tyrosine phosphatase activity and mediates inhibition of particulate guanylate cyclase. Biochem Biophys Res Comun 1992; 183: 2060–211.CrossRefGoogle Scholar
  2. Chaki S, Inagami T: A newly found angiotensin II receptor subtype mediated cyclic GMP formation in differentiated Neuro-2A cells. Eur J. Pharmacol 1992; 225: 355–356.PubMedCrossRefGoogle Scholar
  3. Chang RSL, and Lotti VJ: Two distinct angiotensin II receptor binding sites in rat adrenal revealed by new selective nonpeptide ligands. Mol Pharmacol 1990; 29: 347–351.Google Scholar
  4. Chiu AT, Herblin WF, McCall DE, Ardecky RJ, Carini DJ, Duncia JV, Pease LJ, Wong PC, Wexler RR, Johnson AL, Timmermans PBMWW: Identification of angiotensin II receptor subtypes. Biochem Biophys Res Commun 1989; 165: 196–203.PubMedCrossRefGoogle Scholar
  5. Chiu AT, Roscoe WA, McCall DE, Timmermans PBMWM: Angiotensin II — 1 receptors mediate both vasoconstrictor and hypertrophic responses in rat aortic smooth muscle cells. Receptor 1991; 1:33–40.Google Scholar
  6. Dudley DT, Hubbell SE, Summerfeit RM: Characterization of angiotensin H(T2) binding sites in R3T3 cells. Mol Pharmacol 1991; 40: 360–367.PubMedGoogle Scholar
  7. Elton TS, Stephan CC, Taylor GR, Kimball MG, Martin MM, Durand JN and Oparil S: Isolation of two distinct type I angiotensin II receptor genes. Biochem Biophys Res Commun 1992; 184: 1067–1073.PubMedCrossRefGoogle Scholar
  8. Florio T, Pan MMMG, Newman G, Hershberger RE, Civelli O and Stork PJS: Dopaminergic inhibition of DNA synthesis in pituitary tumor cells is associated with phosphotyrosine phosphatase. J Biol Chem 1992; 267: 24169–24172.PubMedGoogle Scholar
  9. Furuta H, Guo D-F, Inagami T: Molecular cloning and sequencing of the gene encoding human angiotensin II type 1 receptor. Biochem Biophys Res Commun 1992; 183: 8–13.PubMedCrossRefGoogle Scholar
  10. Iwai N, Inagami T: Identification of two subtypes in the rat type 1 angiotensin II receptor. FEBS Lett 1992; 298: 257–260.PubMedCrossRefGoogle Scholar
  11. Iwai N, Yamano Y, Chaki S, Konishi F, Bardhan S, Tibbetts C, Sasaki K, Hasegawa M, Matsuda Y, and Inagami T: Rat angiotensin II receptor: cDNA sequence and regulation of the gene expression. Biochem Biophys Res Commun 1992; 177: 299–304.CrossRefGoogle Scholar
  12. Jackson TR, Blair LAC, Marshal J, Goedert M and Hanley MR: The mas oncogene encodes an angiotensin receptor. Nature 1988; 335: 437–440.PubMedCrossRefGoogle Scholar
  13. Kakar SS, Sellers JC, Devor DC, Musgrove LC and Neil JD: Angiotensin type-a receptor subtype cDNAs: Differential tissue expression and hormonal regulation. Biochem Biophys Res Commun 1992; 184: 1067–1073.CrossRefGoogle Scholar
  14. Kambayashi Y, Bardhan S and Inagami T: Peptide growth factors markedly decrease the ligand binding of angiotensin II type 2 receptor in rat cultured vascular smooth muscle cells. Biochem Biophys Res Commun 1993(a); 194:478–482.PubMedCrossRefGoogle Scholar
  15. Kambayashi Y, Bardhan S, Takahashi K, Tsuzuki S, Inui H, Hamakubo T and Inagami T: Molecular cloning of a novel angiotensin II receptor isoform involved in phosphotyrosine phosphatase inhibition. J Biol Chem 1993(b); 268:24543–24546.PubMedGoogle Scholar
  16. Leung KH, Roscoe WA, Smith RD, Timmermans PBMWM and Chiu AT: Characterization of biochemical responses of angiotensin II (AT2) binding site in the rat pheochromocytoma PC12w cells. Eur J Pharmacol. 1992; 227: 63–70.PubMedCrossRefGoogle Scholar
  17. Lewis JL, Serikawa T, Warnock DG: Chromosomal localization of angiotensin II type 1 receptor isoforms in the rat. Biochem Biophys Res Commun. 1993; 194: 677–682.PubMedCrossRefGoogle Scholar
  18. Marrero, MB, Paxton, WG, Duff, JL, Bradford C Berk, and Bernstein, KE: Angiotensin II stimulates tyrosine phosphorylation of phospholipase C-γ1 in vascular smooth muscle cells. J Biol Chem. 1994; 269: 10935–10939.PubMedGoogle Scholar
  19. Mukoyama M, Nakajiima M, Horicuchi M, Sasamura H, Pratt RE and Dzau JV: Expression cloning of type 2 angiotensin II receptor reveals a unique class of seven-transmembrane receptors. J Biol Chem. 1993; 268: 24539–24542.PubMedGoogle Scholar
  20. Murphy TJ, Alexander RW, Griendling KK, Runge MS, Bernstein KE: Isolation of a cDNA encoding the vascular type-1 angiotensin II receptor. Nature 1991; 351: 233–236.PubMedCrossRefGoogle Scholar
  21. Ohnishi J, Ishido M, Shibata T, Inagami T, Murakami K and Miyazaki H: The rat angiotensin II AT1A receptor couples with three different signal transduction pathways. Biochem Biophys Res. Commun 1992; 186: 1094–1101.PubMedCrossRefGoogle Scholar
  22. Pan MG, Florio T and Stork PJS: G-protein activation of hormone stimulated phosphatase in human tumor cells. Science 1992; 256: 1215–1217.PubMedCrossRefGoogle Scholar
  23. Peach MJ: Molecular actions of angiotensin. Biochem Pharmacol 1981; 30:2745–2751.PubMedCrossRefGoogle Scholar
  24. Pucell AG, Hodges JC, Sen I, Bumpus FM and Husain A: Biochemical properties of the ovarian granulosa cell type-2 angiotensin II receptors. Endocrinology 1991; 128: 1947–1959.PubMedCrossRefGoogle Scholar
  25. Rens-Domiano S, Law SF, Yamada Y, Seino S, Bell GI and Reisine T: Pharmacological properties of two cloned somatostatin receptors. Mol Pharmacol 1992; 42: 28–34.PubMedGoogle Scholar
  26. Sandberg K, Ji H, Clark AJI, Shapira H and Catt KJ, et al.: Cloning and expression of a novel angiotensin II receptor subtype. J Biol Chem 1992; 267: 9455–9458.PubMedGoogle Scholar
  27. Sasaki K, Yamano Y, Bardhan S, Iwai N, Murray JJ, Hasegawa M, Matsuda Y and Inagami T: Cloning and expression of a complementary DNA encoding a bovine adrenal angiotensin II type 1 receptor. Nature 1991; 351: 230–233.PubMedCrossRefGoogle Scholar
  28. Sasamura H, Hein L, Krieger JE, Pratt RE, Kobilka BK and Dzau VJ: Cloning, characterization and expression of two angiotensin receptor (AT-1) isoforms from the mouse genome. Biochem Biophys Res Commun 1992; 185: 253–259.PubMedCrossRefGoogle Scholar
  29. Seed B: An LFA-3 cDNA encodes a phospholipid-linked membrane protein homologous to its receptor CD2. Nature 1987; 329:840–842.PubMedCrossRefGoogle Scholar
  30. Speth RC, Kim KH: Discrimination of two angiotensin II receptor subtypes with a selective agonist analogue of angiotensin II p-aminophenylalamine6 angiotensin II. Biochem Biophys Res Commun 1990; 169: 997–1006.PubMedCrossRefGoogle Scholar
  31. Szpirer C, Riviere M, Szpirer J, Leven G, Guo D-F, Iwai N, and Inagami T: The human and rat angiotensin II receptor genes: chromosomal localization of the type 1 genes. Hypertension 1993; 11: 919–925.CrossRefGoogle Scholar
  32. Takahashi K, Bardhan S, Kambayashi Y, Shirai H and Inagami T: Protein tyrosine phosphatase inhibition by angiotensin II in rat pheochromocytoma cells in vivo via AT2 receptor. Biochem Biophys Res Commun (in press).Google Scholar
  33. Takayanagi R, Ohnaka K, Sakai Y, Nakao R, Yanase T, Haji M, Inagami T, Furuta H, Guo D-F, Nakamuta M, Nawata H: Molecular cloning, sequencing analysis and expression of a cDNA encoding human type-1 angiotensin II receptor. Biochem Biophys Res Commun 1992; 183: 910–916.PubMedCrossRefGoogle Scholar
  34. Webb ML, Lieu ECK, Cohen RB, Hedberg A, Bogosian EA, Monshizadegau H, Mollogy C, Serafino R, Moreland S, Murphy TJ and Dickinson KEJ: Molecular characterization of angiotensin II type II receptor in rat pheochromocytoma cells. Peptides 1992; 13: 499–508.PubMedCrossRefGoogle Scholar
  35. Whitebread S, Mele M, Kamber B, and deGaspero M: Preliminary biochemical characterization of two angiotensin II receptor subtypes. Biochem Biophys Res Commun 1989; 163: 284–291.PubMedCrossRefGoogle Scholar
  36. Yoshida H, Kakinuma J, Guo D-F, Furuta H, Iwai N, van der Meer-de-Jong R, Inagami T and Ichikawa I: Analysis of the evolution of angiotensin II type II receptor gene in mammals (mouse, rat, bovine and human). Biochem Biophys Res Commun 1992; 186: 1042–1049.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1995

Authors and Affiliations

  • Tadashi Inagami
    • 1
  • Yoshiaki Yamano
    • 1
  • Smriti Bardhan
    • 1
  • Shigeyuki Chaki
    • 1
  • Deng-Fu Guo
    • 1
  • Kenji Ohyama
    • 1
  • Yoshikazu Kambayashi
    • 1
  • Kyoko Takahashi
    • 1
  • Toshihiro Ichiki
    • 1
  • Satoshi Tsuzuki
    • 1
  • Hua Tang
    • 1
  1. 1.Department of BiochemistryVanderbilt University School of MedicineNashvilleUSA

Personalised recommendations