Calcium Regulates the Differentiation of Vitamin A-Responsive Human Papillomavirus-Immortalized Cervical Keratinocytes, But Not the Expression of the Papillomavirus E6 and E7 Oncogenes

  • C. K. Choo
  • E. A. Rorke
  • R. L. Eckert
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 375)

Abstract

Transcription of the papillomavirus virus open reading frame encoding oncogenes E6 and E7 is necessary for cell immortalization and is known to be regulated as a function of cell differentiation in cervical tumors in vivo. Thus, it is important to understand how exogenous factors regulate transcription from the HPV P97 promoter. Vitamin A and other retinoids are important dietary agents that regulate normal ectocervical epithelial cell differentiation. Moreover, these agents have also been shown to be clinically useful in reducing the mass of cervical tumors. In a previous study supported by the AICR, we examined the effects of vitamin A and other retinoids on the differentiation of an HPV16-immortalized cervical cell line, ECE16-1. We showed that HPV16-immortalization sensitizes HPV16 transformed cells to the effects of retinoids [Agarwal et al., Cancer Res 51: 3982–3989 (1991)].

Keywords

Cell Immortalization Cervical Tumor Cervical Cell Tumor Cell Differentiation Cervical Cell Line 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • C. K. Choo
    • 1
  • E. A. Rorke
    • 1
  • R. L. Eckert
    • 1
  1. 1.Departments of Physiology and Biophysics, Dermatology, Reproductive Biology, Environmental Health Sciences and BiochemistryCase Western Reserve University School of MedicineClevelandUSA

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