Wide Range Transmitter Sensitivities of a Crustacean Chloride Channel

  • Hanns Hatt
  • Ch. Franke
Chapter
Part of the NATO ASI Series book series (NSSA, volume 188)

Abstract

Crustacean neuromuscular junctions use glutamate as the excitatory transmitter and GABA as the inhibitory transmitter. Excitatory, glutamate-activated channels have been studied in locust and crayfish by recording single channel currents (Patlack et al., 1979; Cull-Candy et al., 1980; Franke et al., 1983). Recently, the recording technique has been improved to the point at which GΩ-seals can be obtained, and these have been used to characterize the excitatory, glutamate-activated channels in muscle fibers of crayfish (Franke et al., 1987; Hatt et al., 1988a) and locust (Dudel et al., 1988). The channel has a high conductance of about 100 pS and single openings are very short: on the average 0.2 to 0.3 ms. At high glutamate concentrations, channel openings are grouped in bursts, the number of openings per burst and burst duration increasing when glutamate concentration rises from 0.2 to 10 mM. The channel described above is located at the excitatory synapses of muscle fibers of crayfish.

Keywords

Channel Opening Chloride Channel Stomach Muscle Single Channel Current Nicotinic Agonist 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Barnard, E. A., Darlison, M. G., and Seeburg, P., 1987, Molecular biology of GABAA receptor: the receptor/channel superfamily. Trends Neurosci. 10:502–509.CrossRefGoogle Scholar
  2. Cull-Candy, S. G., Miledi, R., and Parker, I., 1980, Single glutamate-activated channels recorded from locus muscle fibres with perfused patch-clamp electrodes. J. Physiol. (Lond.) 321:195–210.Google Scholar
  3. Dudel, J., 1977, Dose-response curve of glutamate applied by superfision to crayfish muscle synapses. Pflügers Arch. 368:49–54.PubMedCrossRefGoogle Scholar
  4. Dudel, J., and Kuffler, S. W., 1961, Presynaptic inhibition at the crayfish neuromuscular junction. J. Physiol. (Lond.) 155:543–562.Google Scholar
  5. Dudel, J., Finger, W., and Stettmeier, H., 1980, Inhibitory synaptic channels activated by λ-aminobutyric acid (GABA) in crayfish muscle. Pflügers Arch. 387:143–151.PubMedCrossRefGoogle Scholar
  6. Dudel, L., Franke, Ch., Hatt, H., Ramsey, R. L., and Usherwood, P. N. R., 1988, Rapid activation and desensitization by glutamate or excitatory, cation-selective channels in locust muscle. Neurosci. Lett. 88:33–38.PubMedCrossRefGoogle Scholar
  7. Fatt, P., and Katz, B., 1953, The effect of inhibitory nerve impulses on a crustacean muscle fibre. J. Physiol. (Lond.) 121:374–389.Google Scholar
  8. Franke, Ch., Dudel, J., and Finger, W., 1983, Single synaptic channels recorded at glutamate sensitive patches on a crayfish muscle. Neurosci. Lett. 42:7–12.PubMedCrossRefGoogle Scholar
  9. Franke, Ch., Hatt, H., and Dudel, J., 1986a, The excitatory glutamate-activated channel recorded in cellattached and excised patches from the membranes of tail, leg and stomach muscles of crayfish. J. Comp. Physiol. A. 159:579–589.CrossRefGoogle Scholar
  10. Franke, Ch., Hatt, H., and Dudel, J., 1986b, The inhibitory chloride channel activated by glutamate as well as-amino-butyric acid (GABA). Single channel recordings from crayfish muscle. J. Comp. Physiol. A. 159:591–609.CrossRefGoogle Scholar
  11. Franke, Ch., Hatt, H., and Dudel, J., 1987, Liquid filament switch for ultra-fash exchanges of solutions at excised patches of synaptic membrane of crayfish muscle. Neurosci. Lett. 77:199–204.PubMedCrossRefGoogle Scholar
  12. Hatt, H., Franke, Ch., and Dudel, J., 1988a, Ionic permeabilities of L-glutamate activated, excitatory synaptic channel in crayfish muscle. Pflügers Arch. 411:8–16.PubMedCrossRefGoogle Scholar
  13. Hatt, H., Franke, Ch., and Dudel, J., 1988b, Calcium dependent gating of the L-glutamate activated, excitatory synaptic channel on crayfish muscle. Pflügers Arch. 411:17–26.PubMedCrossRefGoogle Scholar
  14. King, W., and Carpenter, D. O., 1987, Distinct GABA and glutamate receptors may share a common channel in Aplysia neurons. Neurosci. Lett. 82:343–348.PubMedCrossRefGoogle Scholar
  15. Onodera, K., and Takeuchi, A., 1976, Inhibitory postsynaptic current in voltage-clamped crayfish muscle. Nature 263:153–154.PubMedCrossRefGoogle Scholar
  16. Onodera, K., and Takeuchi, A., 1979, An analysis of the inhibitory postsynaptic current in the voltage-damped crayfish muscle. J. Physiol (Lond.) 286:265–282.Google Scholar
  17. Otsuka, M., Iversen, L. L., Hall, Z. W., and Kravitz, E. A., 1966, Release of gamma-amino butyric acid from inhibitory nerves of lobster. Proc. Nad. Acad. Sci. U.SA. 56:1110–1115.CrossRefGoogle Scholar
  18. Patlak, J. B., Gration, K. A. F., and Usherwood, P. N. R., 1979, Single glutamate activated channels in locust muscle. Nature 278:643–645.PubMedCrossRefGoogle Scholar
  19. Smart, T. G., and Constanti, A., 1986, Studies on the mechanism of action of Picrotoxin and other convulsants at the crustacean muscle GADA receptor. Proc. R. Soc. Lond. B 227:191–216.CrossRefGoogle Scholar
  20. Takeuchi, A., and Takeuchi, N., 1969, A study of the action of picrotoxin on the inhibitory neuromuscular junction of the crayfish. J. Physiol. (Lond.) 205:377–391.Google Scholar
  21. Takeuchi, A., and Takeuchi, N., 1971a, Anion interaction at the inhibitory postsynaptic membrane of the crayfish neuromuscular junction. J. Physiol. (Lond) 212:337–351.Google Scholar
  22. Takeuchi, A., and Takeuchi, N., 1971b, Variations in the permeability properties of the inhibitory postsynaptic membrane of the crayfish neuromuscular junction when activated by different concentrations of GABA. J. Physiol. (Lond.) 217:341–358.Google Scholar
  23. Zufall, F., Franke, Ch., and Hatt, H., 1988, Acetylcholine activates a chloride channel as well as glutamate and GABA. Single channel recordings from crayfish stomach and opener muscles. J. Comp. Physiol. A 163:609–620.PubMedCrossRefGoogle Scholar
  24. Zufall, F., Franke, Ch., and Hatt, H., 1989, The insecticide avermectin B1a activates a chloride channel in crayfish muscle membrane. J. exp. Biol. 142:191–205.PubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • Hanns Hatt
    • 1
  • Ch. Franke
    • 1
  1. 1.Physiologisches InstitutTechnischen Universität MünchenMünchen 40Federal Republic of Germany

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