Urolithiasis pp 549-551 | Cite as

Microdetermination of Urinary Constituents by Vertical Light-Path Photometry in Microplates

  • W. Achilles
  • C. Schalk
  • J. Berwernick
  • G. Rodeck

Abstract

Automated photometric measurements in microtiter plates have been used for some years in our laboratory in order to determine kinetic parameters of crystal growth in gels (1). We have now applied vertical light-path photometry in 96-well microplates to quantify various soluble constituents of urine and serum which are relevant to diagnosis and basic research of urinary stone formation.

Keywords

Uric Acid Photometric Measurement Soluble Constituent Urinary Stone Formation Calcium Stone Formation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    W Achilles, Crystallization in gel matrices: a new experimental model of calcium stone formation, Contr. Nephrol. 58: 59 (1987).Google Scholar
  2. 2.
    PF Wright, DEJ Gall, and WA Kelly, Effect of meniscus formation and duplicate sample placement configurations on the variability of measurement by three microtiter plate photometers, J. Immun. Meth. 81: 83 (1985).CrossRefGoogle Scholar
  3. 3.
    JDE Young, CC Liu, LG Leong, A Damiano, PM Persechini, and ZA Cohn, An automated colorimetric microassay system for rapid and quantitative determination of soluble enzymatic activities, Biotechniques 5: 572 (1987).Google Scholar

Copyright information

© Springer Science+Business Media New York 1989

Authors and Affiliations

  • W. Achilles
    • 1
  • C. Schalk
    • 1
  • J. Berwernick
    • 1
  • G. Rodeck
    • 1
  1. 1.Urologie/Klin. ForschungUniversitätsklinikum MarburgMarburg/LahnGermany

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