Hepoxilin A3 Induces Vascular Permeability in the Skin
Hepoxilins are 11S,12S-epoxy-8(R,S)-hydroxy metabolites of arachidonic acid formed through the 12-lipoxygenase pathway from the intermediate, 12S-HPETE (1, 2). Evidence to date shows that the hepoxilins are formed through an Fe+3 catalysed rearrangement of 12-HPETE, which although retaining both oxygen atoms of 12-HPETE through an intramolecular isomerisation, the hydroxyl group at carbon 8 is racemic (3, 4). During the past few years we have been interested in profiling the biological properties of the 8R and 8S isomers of HxA3. The present report demonstrates that the hepoxilins are active in causing vascular permeability in the rat skin, and although they are not powerful on their own, they greatly potentiate the actions of subthreshold concentrations of bradykinin.
KeywordsArachidonic Acid Vascular Permeability Evans Blue Dorsal Skin Hydroxy Metabolite
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