Metabolism of Arachidonic Acid by Purified Lung Epithelial Cells

  • Pierre Sirois
  • Stéphane Prié
  • Johanne Laporte
  • Karim Maghni
  • Pierre Borgeat
Part of the GWUMC Department of Biochemistry Annual Spring Symposia book series (GWUN)

Abstract

The airway epithelium is composed of a number of cell types with highly selective functions. The mucus cells, the Clara cells and, to a certain extent, the type II pneumocytes have major secretory roles. Whereas mucus cells are responsible for the secretion of the mucoproteins covering large airways (St. George et al., 1988), type II pneumocytes (Bantenburg and Val Go1de, 1979; King, 1982) and Clara cells (Niden, 1967) secrete surfactant which cover small airways and maintain surface tension. The ciliated cells allow mucociliary transport (Gail and Lenfant, 1983) and the basal cells are involved in the attachment of columnar cells to the lamina propria (Evans and Plopper, 1988). Clara cells are the primary sites of pulmonary defense mechanism against toxicants and are involved in the activation and detoxification of xenobiotics by their active P-450 cytochrome monooxygenase pathway (Boyd, 1977). In addition, epithelial cells from various species including man, rabbit and rat were shown to release arachidonic acid metabolites which modulate mucus secretion (Gail and Lenfant, 1983) and airway caliber (Sirois et al., 1987). The purpose of the following investigation was to study the metabolism of arachidonic acid by purified populations of guinea pig epithelial cells.

Keywords

Arachidonic Acid Airway Epithelial Cell Ciliated Cell Mucus Cell Clara Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bantenburg, J.J. and Van Golde, L.M.G., 1979, Formation of pulmonary surfactant in whole lung and in isolated type II alveolar cells, Rev. Perinat. Med. 3:73.Google Scholar
  2. Borgeat, P., Picard, S., Vallerand, P., Bourgoin, S., Odeimat, A., Sirois, P. and Poubelle, P.E., 1990, Automated on-line extraction and profiling of lipoxygenase products of arachidonic acid by high performance liquid chromatography, in: “Methods in Enzymology Arachidonate related lipid mediators”, R.C. Murphy and F. Fitzpatrick, eds., Academic Press, New York.Google Scholar
  3. Boyd, M.R., 1977, Evidence for the Clara cell as a site of cytochrome P-450 dpendent mixed function oxydase activity in lung, Nature, 269: 713–715.PubMedCrossRefGoogle Scholar
  4. Chauncey, J.B., Peters-Golden, M. and Simon, R.H., 1988, Arachidonic acid metabolism by rat elveolar epithelial cells, Lab. Invest. 58:133.Google Scholar
  5. Churchill, L., Chilton, F.H., Resau, J.H., Bascom, R., Hubbard, W.C. and Proud, D., 1989, Cyclooxygenase metabolism of endogenous arachidonic acid by cultured human tracheal epithelial cells, Am. Rev. Respir. Dis. 140:449–459.Google Scholar
  6. Dobbs, L.G., Gonzalez, R. and Williams, M.C., 1986, An improved method for isolating type II cells in high yield and purity, Am.Rev. Respir. Dis. 134:141–145.Google Scholar
  7. Evans, M.J. and Plopper C.G., 1988, The role of basal cells in adhesion of columnar epithelium to airway basement membrane, Am. Rev., Respir. Dis. 138:481–483.Google Scholar
  8. Feinmark, J.J. and Cannon, J.J., 1986, Endothelial cell leukotriene C4 synthesis results from intercellular transfer of leukotriene A4 synthesized by polymorphonuclear leukocytes, J.Biol. Chem. 261:16466.Google Scholar
  9. Gail, D.B. and Lenfant, C.J.M., 1983, Cells of the lung: biology and clinical implications, Am.Rev. Respir. Dis. 127:366–387.Google Scholar
  10. Gilfillan, A.M. and Rooney, S.A., 1985, Arachidonic acid metabolites stimulate phosphophatidylcholine secretion in primary culture of type II pneumocytes, Biochem. Biophys. Acta. 833:336.Google Scholar
  11. Graeber, J.E., Walenga, R.W., Ulane, R.E. and Stuart, M.J., 1982, Metabolism of 14Carachidonic acid by type II alveolar epithelial cells in primary culture, Pediat. Res., 16: 350A.Google Scholar
  12. Holtzman, M.J., Hasnsbrough, J.R. Rosen, G.D. and Turk, J., 1988, Uptake, release and novel species-dependent oxygenation of arachidonic acid in human and animal airway epithelial cells, Biochim. Biophys. Acta. 963: 401–413.Google Scholar
  13. Laporte, J., Hallée, A. Maghni, K., Robidoux, C., Borgeat, P. and Sirois, P., 1991, Metabolism of arachidonic acid by guineau pig Clara cells Protaglandins 41: 263.Google Scholar
  14. Maghni, K., Robidoux, C., Laporte, J., Hallée, A. and Sirois, P., 1990, Release of prostaglandins and thromboxanes by guinea pig isolated type II pneumocytes, Prostaglandins 40: 217.Google Scholar
  15. Marcus, A.J., Brockman, M.J., Sagier, B., Ullman, H.L., Islam, K.J., Serhan, C.N., Rutherford, L.E., Korchak, H.M. and Weissman, G., 1982, Formation ofGoogle Scholar
  16. leukotrienes and other hydroxy acids during platelet-neutrophils interaction in vivo, Biochem. Biophys. Res. Commun. 109:130.Google Scholar
  17. Niden, A.H., 1967, Bronchiolar and large alveolar cell in pulmonary phospholipid metabolism, Science, 158: 1323–4.PubMedCrossRefGoogle Scholar
  18. Oryazum, M.J. and Clements, J.A., 1978, Control of lung surfactant by ventilation, adrenergic mediators, and prostaglandins in the rabbit, Am.Rev. Respir. Dis. 117:879.Google Scholar
  19. Pelé, J.P., Robidoux, C. and Sirois, P., 1989, Guinea pig lung cells. Method of isolation and partial purification, identification, ultrastructure and cell count, Inflammation 13: 103.Google Scholar
  20. Pradelles, P., Grassi, J. and Maclouf, J., 1985, Enzyme immunoassay of eicosanoids using acetylcholine esterase as label: An alternative to radioimmunoassay, Anal. Chem., 57: 1170.Google Scholar
  21. Prié, S., Cadieux, A. and Sirois, P., 1990, Removal of guinea pig bronchial and tracheal epithelium potentiates the contractions to leukotrienes and histamine, Eicosanoids, 3: 29–37.PubMedGoogle Scholar
  22. St. George, J.A., Harkema, J.R., Hyde, D.M. and Plopper, C.G., 1988, Cell populations and structure-function relaitonships of cells in the airways, in: “Toxicology of the lung”, D.E. Gardner, J.D. Crapo and E.J. Massaro, eds., Raven Press, New York.Google Scholar
  23. Sirois, P., Harczy, M., Maclouf, J., Pradelels, P., Braquet, P. and Borgeat, P., 1987, Lipid mediators in lung anaphylaxis: Kinetics of their release and modulation by selected drugs, in: “Lipid mediators in immunology of burn and sepsis”, P. Braquet, ed., Plenum Press.Google Scholar
  24. Taylor, L., Polgar, P., McAteer, J.A. and Douglas, W.H.J., 1979, Prostaglandin production by type II alveolar epithelial cells, Biochem. Biophys. Acta. 572:502.Google Scholar
  25. Xu, G.L., Sivrajah, K. Wu. R., Nettesheim, P. and Eling, T., 1986, Biosynthesis of prostaglandins by isolated and cultured airway epithelial cells, Exp. Lung Res. 10:101–114.Google Scholar

Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Pierre Sirois
    • 1
  • Stéphane Prié
    • 1
  • Johanne Laporte
    • 1
  • Karim Maghni
    • 1
  • Pierre Borgeat
    • 2
  1. 1.Department of Pharmacology Faculty of MedicineUniversity of SherbrookeSherbrooke(P.Q.)Canada
  2. 2.Inflammation et Immunologie-Rhumatologie, CHULQuebecCanada

Personalised recommendations