Genetics of Reoviruses
The ultimate goal of genetic analysis of the mammalian reoviruses is to gain a detailed understanding of the structure and function of the genome and each of the viral polypeptide products. The early phases of genetic analysis were directed toward the isolation and physiological and genetic characterization of a collection of genetic markers, in this case, conditional lethal mutants of the temperature-sensitive type. More recent studies, particularly those since 1975, have emphasized the use of recombinant viruses generated during mixed infection of tissue-culture cells with pairs of viruses, most often of two different reovirus serotypes.
KeywordsMixed Infection Genome Segment Mutant Group Nonpermissive Temperature Suppressor Mutation
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