Induction and Selection of Atrazine Resistance Variants from Tobacco Cell Cultures

  • Wen Chung Wang
  • G. B. Collins
Chapter
Part of the Basic Life Sciences book series (BLSC, volume 32)

Abstract

Photomixotrophic cell cultures of Nicotiana tabacum L. (var. Wisconsin 38) were obtained by repeated selection of cell cultures initially established from hypocotyl expiants. Attempts to induce photomixotrophic cell cultures of N. tabacum L. var. Ky 14 were not successful. Calli derived from Wisconsin 38 hypocotyls regularly produced a greening response and were used for optimizing the growth conditions for photoautotrophic and photomixotrophic cultures. Two light intensities (67 and 7.6 μE/M2 sec) and 4 sucrose concentrations (0, 0.5, 1, and 2%) were tested for optimizing chlorophyll synthesis and callus growth rate. Calli growing on media containing 0.5% sucrose gave better chlorophyll synthesis and chlorophyll a/b ratios at high light intensity than did other treatments.

Keywords

Sucrose Concentration Nicotiana Tabacum High Light Intensity Chlorophyll Synthesis Hypocotyl Explants 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • Wen Chung Wang
    • 1
  • G. B. Collins
    • 1
  1. 1.Department of AgronomyUniversity of KentuckyLexingtonUSA

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