Morphogenetic Response from Orchardgrass Pistils

  • D. D. Songstad
  • B. V. Conger
Chapter
Part of the Basic Life Sciences book series (BLSC, volume 32)

Abstract

Unpollinated orchardgrass (Dactylis glomerata L.) pistils from an embryogenic genotype were cultured on a Schenk and Hildebrandt (SH) basal medium supplemented with 3% sucrose and 30 μM 3,6-dichloro-o-anisic acid (dicamba) in the dark at 25°C. The experimental design was a randomized complete block with 6 treatments (1- to 6-week culture periods) and 3 blocks where each experimental unit consisted of 15 pistils. The morphogenetic response was followed at weekly intervals for 6 weeks by stereoscopic and scanning electron microscopy. Root-like protrusions emerged basipetally from approximately 15% of the pistils cultured for one week; however, no plants were regenerated from these structures. Ovary and style regions became swollen after 2 weeks, and embryoids emerged directly from these areas during the third week of culture. These embryoids acted as secondary expiants and initiated calli in 4-week-old cultures. During the fifth and sixth weeks, many embryoids were initiated from the newly formed calli. Three weeks after transfer of these cultures to dicamba-free medium, the embryogenic response was quantified by counting shoots. A significant F value (α = 0.05) for the treatment effects was followed by mean separation through orthogonal contrasts. This revealed no significant difference between the 3-and 4-week treatments.

Keywords

Scanning Electron Microscopy Basal Medium Weekly Interval Experimental Unit Morphological Observation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Copyright information

© Springer Science+Business Media New York 1985

Authors and Affiliations

  • D. D. Songstad
    • 1
  • B. V. Conger
    • 1
  1. 1.Department of Plant and Soil ScienceThe University of TennesseeKnoxvilleUSA

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