Somatic Embryogenesis from Mature Wheat Embryos
Cereal calli which are most responsive to regeneration attempts have been those derived from immature embryos, inflorescences, or leaves. The basis of these regenerative responses appears to be somatic embryogenesis. There is little evidence in the literature of “mature” tissue (e. g., whole caryopses, isolated mature embryos, and expanded leaves) yielding significant numbers of embryoids. This report details the consistent formation in vitro of significant amounts of somatic embryos from either whole caryopses (seeds) or isolated mature embryos of Triticum aestivum (cv. Glennson) cultured on a high (5 mg/1) 2,4-dichlorophenoxyacetic acid (2,4-D) concentration.