Bacteriophage Lambda and Plasmid lacZ Transgenic Mice for Studying Mutations in Vivo

  • Jan Vijg
  • George R. Douglas

Abstract

The demonstration that genomic DNA fragments, including integrated vectors, can be efficiently rescued and cloned only into host-restriction negative bacterial strains, allowed the practical use of transgenic mice equipped with bacterial reporter genes for mutation analysis studies (Gossen and Vijg, 1988; Gossen et al., 1989). This was later confirmed by Kohler et al. (1990). Since then numerous studies with lacZ and/or lacI transgenic mice have demonstrated the power of these model systems to detect spontaneous and induced mutations (Kohler et al., 1991; Myhr, 1991; Suzuki et al., 1993; Gossen and Vijg, 1993; Tinwell et al., 1994; Douglas et al., 1994, 1995a,b).

Keywords

Transgenic Mouse Seminiferous Tubule lacZ Gene Bacteriophage Lambda Selective Plate 
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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Jan Vijg
    • 1
  • George R. Douglas
    • 2
  1. 1.Molecular Genetics Section, Gerontology Division, Department of MedicineBeth Israel Hospital and Harvard Medical SchoolBostonUSA
  2. 2.Mutagenesis Section, Environmental Health DirectorateHealth CanadaOttawaCanada

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