DNA Damage Analysis Using an Automated DNA Sequencer
Advances in biotechnology and molecular genetics have made possible a better understanding of the molecular nature of mutation. For example, the discovery of genetically altered proto-oncogenes and tumor suppressor genes in cancerous cells has led to a better understanding of the links between mutation and cancer. Similarly, the ability to study mutation and mutational specificity in vivo and in vitro has led to an increased appreciation of the mechanisms of mutation and the role that DNA damage and DNA repair play in determining the specificity of mutagenesis. In turn, differences in both the cellular metabolism of exogenous chemicals and DNA repair can at least in part explain tissue, gender, and species specificity of carcinogenesis. We remain, however, a long way off from being able to predict the individual risks implicated with the mutagenic potential of chemical and physical agents. A part of this problem reflects our lack of knowledge of how individual lesions are handled in different tissues and different species against the genetic makeup of an individual.
KeywordsCyclobutane Pyrimidine Dimer Acrylamide Formation Retention Time Shift Salt Front Capillary Electrophoresis Instrument
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