Mapping of Genes Controlling Cold Hardiness on Wheat 5A and its Homologous Chromosomes of Cereals

  • Gabor Galiba
  • Ildiko Kerepesi
  • John W. Snape
  • Jozsef Sutka

Summary

The analysis of monosomic and substitution lines showed that at least ten of the 21 pairs of chromosomes are involved in the control of frost tolerance and winter hardiness in wheat (Triticum aestivum L.). Of particular importance for adaptation to autumn sowing are the genes for vernalization requirement (Vrn). Major genes influencing frost resistance (Fr1) and vernalization requirement (Vrn1) were localized on the long arm of chromosome 5 A. To map the location of these genes, single chromosome recombinant lines were developed from the cross between substitution lines: Chinese Spring(Cheyenne 5A) (CS(Ch5A)) and CS(Triticum spelta 5A). The RFLP data show that although a close genetic linkage exist between the loci Fr1 and Vrn1, they are, nevertheless, separable. The location of Vrn1 suggests that it is homologous to other spring habit genes in related species, particularly to the Sh2 locus on choromosome 7(5H) of barley and to the Sp1 locus on chromosome 5R of rye. The level of abscisic acid (ABA) in plants increases as a result of water deprivation or low temperatures. Our study on CS(Ch) substitution lines showed that chromosome 5A of the frost resistant Ch increased the ABA content in the frost sensitive CS background following cold treatment. The crown ABA accumulation in several of the 5A recombinant lines subjected to polyethylene glycol induced water stress and revealed a close genetic linkage between both Fr1 and Vrn1 genes and the gene regulating ABA production. Studies on different substitution lines also showed that the 5A chromosome is involved in osmotic stress induced amino acid and polyamine and, moreover, in cold stress induced proline accumulation. A gene regulating cold induced sugar accumulation has also been localized on chromosome 5A. Accordingly, gene(s) involved in os-moregulation could also be localized on the chromosome 5A.

Keywords

Substitution Line Frost Resistance Cold Hardiness Frost Tolerance Vernalization Requirement 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Gabor Galiba
    • 1
  • Ildiko Kerepesi
    • 2
  • John W. Snape
    • 3
  • Jozsef Sutka
    • 1
  1. 1.Agricultural Research Institute of the Hungarian Academy of SciencesMartonvasarHungary
  2. 2.Department of Chemistry and BiochemistryJanus Pannonius UniversityPecsHungary
  3. 3.Cereal Research DepartmentJohn Innes CentreColney, NorwichGreat Britain

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