In Planta Metabolic State of Neotyphodium Endophyte Mycelium Assessed Through Use of the GUS Reporter Gene in Combination with Hyphal Enumeration

  • Y. Y. Tan
  • M. Spiering
  • M. J. Christensen
  • K. Saunders
  • J. Schmid

Abstract

We have recently developed methods for the quantitative measurement of B-glucuronidase (GUS) reporter gene activity of transformants of Neotyphodium endophytes during their growth in perennial ryegrass (2). When transformants express the reporter gene constitutively, GUS activity can be used as an indicator of the distribution of in planta endophyte metabolic activity (2). Because the concentration of endophyte metabolic activity in grass tissue is a result of both the concentration of endophyte hyphae and their metabolic state, we have now combined GUS assays with methods for the determination of biovolume of endophyte hyphae in plant sections to assess the metabolic state of the endophyte mycelium.

Keywords

Metabolic State Endophytic Fungus Leaf Sheath Tall Fescue Perennial Ryegrass 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Christensen, M. J., A. Leuchtmann, D. D. Rowan, and B. A. Tapper. 1993. Taxonomy of Acremonium endophytes of tall fescue (Festuca arundinacea), meadow fescue (F. pratensis), and perennial rye-grass (Lolium perenne). Mycolog. Res. 97: 1083–1092.Google Scholar
  2. Herd, S., M. J. Christensen, K. Saunders, D. B. Scott, and J. Schmid. 1996. Quantitative assessment of in planta distribution of metabolic activity and gene expression of endophytic fungi. Microbiology in press.Google Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Y. Y. Tan
    • 1
  • M. Spiering
    • 1
  • M. J. Christensen
    • 2
  • K. Saunders
    • 1
  • J. Schmid
    • 1
  1. 1.Department of Microbiology and GeneticsMassey UniversityPalmerston NorthNew Zealand
  2. 2.Department of Microbiology and GeneticsAgResearch GrasslandsPalmerston NorthNew Zealand

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