Taurine 2 pp 417-425 | Cite as

Nitric Oxide-Evoked [3H]Taurine Release is Mediated by Reversal of the Na+-Dependent Carrier-Mediated Taurine Transport System

  • Seitaro Ohkuma
  • Masahi Katsura
  • Da-Zhi Chen
  • Kinya Kuriyama
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 403)

Summary

The pharmacological characteristics of [3H]taurine release evoked by nitric oxide (NO) were investigated using mouse cerebral cortical neurons in primary culture. N-Methyl-D-aspartate (NMDA) and S-nitroso-N-acetylpenicillamine (SNAP) dose-dependently increased [3H]taurine release from neurons. The NMDA-evoked release of [3H]taurine was reduced to the basal level by Nω-nitro-L-arginine, a NO synthase inhibitor, and MK-801, a noncompetitive antagonist for NMDA receptors. The NMDA- and SNAP-evoked releases of [3H]taurine were completely abolished by hemoglobin, indicating that these [3H]taurine releases were evoked by NO produced by NMDA receptor activation and liberated from SNAP. Withdrawal of Na+ from incubation buffer significantly inhibited the NMDA- and SNAP-induced [3H]taurine releases, whereas removal of Ca2+ produced no alteration in the SNAP-evoked [3H]taurine release. In addition, β-alanine and guanidinoethane sulfonate, antitransporters of the carrier-mediated taurine transport system, reduced the NMDA- and SNAP-evoked releases of [3H]taurine in a dose-dependent manner. These results indicate that the NO-evoked [3H]taurine release from cerebral cortical neurons is mediated by a reversal of the Na+-dependent carrier-mediated taurine transport system.

Keywords

Nitric Oxide NMDA Receptor Incubation Buffer NMDA Receptor Activation Basal Release 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Seitaro Ohkuma
    • 1
  • Masahi Katsura
    • 1
  • Da-Zhi Chen
    • 1
  • Kinya Kuriyama
    • 1
  1. 1.Department of PharmacologyKyoto Prefectural University of MedicineKamikyo-Ku, KyotoJapan

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