Production of Platelet-Activating Factor by Brain Microvascular Endothelial Cells
Vascular endothelial cells produce platelet-activating factor (PAF) in response to various agonists, and endothelial PAF is one of the key molecules regulating the interactions between blood cells and the vascular wall.1) To investigate the functional specificity of the vascular endothelium in brain, we have established pure cultures of porcine brain microvascular endothelial cells. Endothelial cells from porcine aorta were also cultured, and using these cultures we have conducted a comparative study on PAF production. Since endothelial PAF production is closely related to the adherence of white blood cells to the endothelium,1) we also study the adhesion of human polymorphonuclear neutrophils (PMNs) to the cultured cells.
KeywordsMicrovascular Endothelial Cell Aortic Endothelial Cell Brain Endothelial Cell Brain Microvascular Endothelial Cell Extracellular Release
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