Platelet-Activating Factor Acetylhydrolase Activity in Human Follicular Fluid

  • Hisashi Narahara
  • Yuichiro Tanaka
  • Yasushi Kawano
  • Isao Miyakawa
  • John M. Johnston
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 416)

Abstract

Platelet-activating factor (PAF) has been implicated in a number of reproductive processes ranging from ovulation to parturition. To examine the role of PAF in the human periovulatory processes, the PAF-acetylhydrolase (PAF-AH) activity was assayed in the follicular fluid (FF) obtained in conjunction with the in vitro fertilization and embryo transfer (IVF-ET) procedure and the activity related to oocyte maturation. The PAF-AH activity was also related to the concentrations of estradiol (E2) and progesterone (P) in FF. PAF-AH activity was significantly lower in the FFs obtained from follicles of more than 20 mm in diameter. The enzyme activity was significantly lower in the FFs of patients with a successful outcome of their pregnancies. E2 concentrations were negatively correlated with PAF-AH activities in the FFs. No correlation was found between the PAF-AH activity and concentration of P in the FF. Significantly more mature oocytes were recovered in the group who subsequently become pregnant compared to the non-pregnant group. It is suggested that PAF may be increased following follicular maturation. The increase in PAF may contribute to oocyte maturation and to the successful outcome of pregnancy following fertilization. An additional function of the increased PAF in FF may also be the stimulation of the contraction of smooth muscle in the ovary, thereby assisting the extrusion of the oocyte cumulus cell mass and signaling the completion of ovulation.

Keywords

Follicular Fluid Oocyte Maturation Follicular Maturation Luteinizing Granulosa Cell Human Follicular Fluid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    L. Speroff, R.H. Glass, and N.G. Kase, Regulation of the menstrual cycle, in: Clinical Gynecologic Endocrinology and Infertility, L. Speroff, R.H. Glass, N.G. Kase, eds., 4th edition (1989) pp91.Google Scholar
  2. 2.
    L.L. Espey, Ovulation as an inflammatory reaction: a hypothesis, Biol. Reprod. 22: 73 (1980).CrossRefGoogle Scholar
  3. 3.
    F Snyder, Platelet-activating factor and related acetylated lipids as potent biologically active cellular mechanisms, Am. J. Physio1. 259: C697 (1990).Google Scholar
  4. 4.
    P. Braquet, L, T.uqui, T.Y. Shen, and B.B. Vargaftig, Perspectives in platelet-activating factor research, Parmacol. Rev. 39: 97 (1987)Google Scholar
  5. 5.
    S.M. Prescott, G.A. Zimmerman, and T.M. McIntyre, Platelet-activating factor, J. Biol. Chem. 265:17381 (1990);Google Scholar
  6. 6.
    M.R. Elstad, D.M. Stafforini, K.E. Stremler, G.A. Zimmermen, T.M. McIntyre, and S.M. Prescott, The potential role of the PAF acetylhydrolase in human disease, in. Platelet-Activating Factor and Disease, K. Saito, D.J. Hanahan, eds. International Medical Publishers, Tokyo, Japan: 69 (1989).Google Scholar
  7. 7.
    A.O. Abisogun, P. Braquet, and A, Tsafriri, The involvement of platelet-activating factor in ovulation, Science 243: 381 (1989).PubMedCrossRefGoogle Scholar
  8. 8.
    L.L. Espey, N. Tanaka, D.S. Woodard, M.J.K. Harper, and H. Okamura. Decrease in ovarian platelet-activating factor during ovulation in the gonadotropin-primed immature rat, Biol. Reprod. 41: 104 (1989).PubMedCrossRefGoogle Scholar
  9. 9.
    B.M. Alexander, E.A. Van Kirk, and W.J. Murdoch, Secretion of platelet-activating factor by periovulatory ovine follicles, Life Sci 47: 865 (1990).PubMedCrossRefGoogle Scholar
  10. 10.
    M.J. Amiel, J. Testart, and J. Benveniste, Platelet-activating factor-acether is a component of human follicular fluid Fertil Steril 56: 62 (1991).Google Scholar
  11. 11.
    X.-M. Li, N. Sagawa, Y. Ihara, A. Okagaki, H. Hasegawa, K. Inamori, H. Itoh, T. Mori, C. Ban, The involvement of platelet-activating factor in thrombocytopenia and follicular rupture during gonadotropin-induced superovulation in immature rats, Endocrinology 128: 3132 (1991).CrossRefGoogle Scholar
  12. 12.
    B.S. Minhas, Platelet-activating factor treatment of human spermatozoa enhances fertilization potential, Am. J. Obstet. Gynecol. 168: 1314 (1993).PubMedCrossRefGoogle Scholar
  13. 13.
    C. O’Neill, PAF and the establishment of pregnancy, in: Platelet-activating factor and human disease, P.C. Barnes, C.P. Page, P.M. Henson, eds… Blackwell, Oxford. 282–296 (1990).Google Scholar
  14. 14.
    H. Narahara, R.A. Frenkel, and J.M. Johnston, The role of PAF in reproductive biology, in Advances in Molecular Biology-Lipobiology, R. Gross, ed.. JAI Press Inc., Greenwich, CT, in press..Google Scholar
  15. 15.
    R.S. Fan, M.L. Wardlow, C.P. Cox, K.E. Meng, and D.E. Greene, Human serum acid-labile factor in an acyl-hydrolase that inactivates platelet-activating factor, Fed. Proc. 42: 3120 (1983).Google Scholar
  16. 16.
    H. Narahara, Y. Nishioka, and J.M. Johnston, Secretion of platelet-activating factor acetylhydrolase by human decidual macrophages, J. Endocrinol. Metab. 77: 1258–62 (1993).CrossRefGoogle Scholar
  17. 17.
    H. Narahara, R. Frenkel, and J.M. Johnston, Secretion of platelet-activating factor acetylhydrolase (PAF-AH) following phorbol ester-stimulated differentiation of HL-60 cells, Arch. Biochem. Biophys. 301: 275 (1993).CrossRefGoogle Scholar
  18. 18.
    S. Miyaura, N. Maki, W. Byrd, and J.M. Johnston, The hormonal regulation of platelet-activating factor acetylhydrolase activity in plasma, Lipids 26: 1015 (1991).PubMedCrossRefGoogle Scholar
  19. 19.
    K. Yasuda, and J.M. Johnston, The hormonal regulation of platelet-activating factor acetylhydrolase in the rat, Endocrinology 130: 708 (1992).PubMedCrossRefGoogle Scholar
  20. 20.
    K. Yasuda, H. Eguchi, H.Narahara, and J.M. Johnston, Platelet-activating factor: Its regulation in parturition. in: Eicosanoids and Other Bioactive Lipids in Cancer, Inflammation and Radiation Injury, S. Nigam, K.V. Honn, L.J. Marnett, T.L. Walden, eds., Kluwer Academic Publishers, Boston, 727 (1993).CrossRefGoogle Scholar
  21. 21.
    N. Lepage, R Miron, R. Hammings, K.D. Roberts, and J. Langlais, Distribution of lysophaspholipids and metabolism of platelet-activating factor in human follicular and peritoneal fluids J. Reprod. Fertil. 98: 349 (1993).Google Scholar
  22. 22.
    M. Miwa, T. Miyake, T. Yamanaka, J. Sugatani, Y. Suzuki, S. Sakata, Y. Araki, and M. Matsumoto, Characterization of serum platelet-activating factor (PAF) acetylhydrolase. Correlation between deficiency of serum PAF acetylhydrolase and respiratory symptoms in asthmatic children J. Clin. Invest. 82: 1983 (1988).PubMedCrossRefGoogle Scholar
  23. 23.
    J.A. Loukides, R.A. Loy, R. Edwards, J. Hong, I. Visintin, and M.L. Polan, Human follicular fluid contain tissue macrophages, J. Clin. Endocrinol. Metab. 71: 1363 (1990).PubMedCrossRefGoogle Scholar
  24. 24.
    R. Nakayama, K. Yasuda, T. Okumura, and K. Saito, Effect of 17ß-estradiol on PAF and prostaglandin levels in oophorectomized rat uterus Biochem. Biophys. Acta 1085: 235 (1991).CrossRefGoogle Scholar
  25. 25.
    J. Rabinovici, and M.J. Angle, Platelet-activating factor induces progesterone secretion and changes in morphological appearance on luteinizing granulosa cells in vitro,. Fertil. Steril. 55: 1106 (1991).PubMedGoogle Scholar
  26. 26.
    Y. Takehara, A.M. Dharmarajan, G. Kaufman, E.E. Wallach, Effect of interleukin-1 ß on ovulation in the in-vitro perfused rabbit ovary, Endocrinology 134: 1788 (1994).PubMedCrossRefGoogle Scholar
  27. 27.
    H. Narahara, and J.M. Johnston, Effects of endotoxins and cytokines on the secretion of platelet-activating factor-acetylhydrolase by human decidual macrophages, Am. J. Obstet. Gynecol. 169; 531 (1993).PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Hisashi Narahara
    • 1
    • 2
  • Yuichiro Tanaka
    • 1
  • Yasushi Kawano
    • 1
  • Isao Miyakawa
    • 1
  • John M. Johnston
    • 2
  1. 1.Department of Obstetrics and GynecologyOita Medical UniversityOita-gun, OitaJapan
  2. 2.Departments of Biochemistry and Obstetrics-Gynecology and The Cecil H. and Ida Green Center for Reproductive Biology Sciences Southwestern Medical CenterUniversity of TexasDallasUSA

Personalised recommendations