Taurine 3 pp 339-345 | Cite as

Regulation of Taurine Biosynthesis and Its Physiological Significance in the Brain

  • Jang-Yen Wu
  • Xiao Wen Tang
  • John V. Schloss
  • Morris D. Faiman
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 442)

Abstract

Cysteine sulfinic acid decarboxylase (CSAD), the rate-limiting enzyme in taurine biosynthesis, was found to be activated under conditions that favor protein phosphorylation and inactivated under conditions favoring protein dephosphorylation. Direct incorporation of 32P into purified CSAD has been demonstrated with [γ32P]ATP and PKC, but not PKA. In addition, the 32P labeling of CSAD was inhibited by PKC inhibitors suggesting that PKC is responsible for phosphorylation of CSAD in the brain. Okadaic acid had no effect on CSAD activity at 10 μM suggesting that protein phosphatase-2C (PrP-2C) might be involved in the dephosphorylation of CSAD. Furthermore, it was found that either glutamate- or high K+-induced depolarization increased CSAD activity as well as 32P-incorporation into CSAD in neuronal cultures, supporting the notion that the CSAD activity is endogenously regulated by protein phosphorylation in the brain. A model to link neuronal excitation, phosphorylation of CSAD and increase in taurine biosynthesis is proposed.

Keywords

Okadaic Acid Synaptosomal Fraction Neuronal Stimulation Calf Intestine Phosphatase Cysteine Sulfinic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Jang-Yen Wu
    • 1
  • Xiao Wen Tang
    • 1
  • John V. Schloss
    • 2
  • Morris D. Faiman
    • 3
  1. 1.Department of Physiology and Cell BiologyUniversity of KansasLawrenceUSA
  2. 2.Department of Medicinal ChemistryUniversity of KansasLawrenceUSA
  3. 3.Department of Pharmacology and ToxicologyUniversity of KansasLawrenceUSA

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