Taurine 3 pp 261-268 | Cite as

Molecular Cloning and Functional Expression of an LLC-PK1 Cell Taurine Transporter that is Adaptively Regulated by Taurine

  • Xiaobin Han
  • Andrea M. Budreau
  • Russell W. Chesney
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 442)

Abstract

Studies have shown that the renal tubular epithelium adapts to alterations in the sulfur amino acid composition of the diet. The renal adaptive response has been described in man, mouse, rat, dog, and pig. The observed phenomenon involves increased or decreased initial rate activity of the NaCl-dependent taurine transporter at the brush border membrane surface of the proximal tubule following dietary manipulation of taurine. A cDNA encoding a taurine transporter has been isolated from LLC-PK1 cells, designated pTAUT, and its functional properties have been examined in Xenopus laevis oocytes. The nucleotide sequence of the clone predicts a 621-amino acid protein with about 90% homology to other cloned taurine transporter cDNAs. When expressed in oocytes the transporter displays a Km of 25 μM and is dependent on the presence of external sodium and chloride, characteristics similar to taurine uptake by LLC-PK1 cells. The abundance of pTAUT mRNA and protein were up-regulated in cells cultured in taurine-free medium as compared with cells cultured in medium containing 500 μM taurine. Activation of PKC by PMA had no effect on adaptive regulation of pTAUT mRNA and protein, indicating that down-regulation of LLC-PK1 cell taurine transport activity by PMA occurs at the post-translational level.

Keywords

MDCK Cell Xenopus Laevis Oocyte Adaptive Regulation Taurine Transporter Taurine Content 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Xiaobin Han
    • 1
  • Andrea M. Budreau
    • 1
  • Russell W. Chesney
    • 1
  1. 1.Department of PediatricsUniversity of Tennessee and the Crippled Children’s Foundation Research Center at Le Bonheur Children’s Medical CenterMemphisUSA

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