A New Assay to Asses Aneuploidy in Human-Hamster Embryos

  • Immaculada Ponsa
  • Laura Tusell
  • Ricard Álvarez
  • Anna Genescà
  • Rosa Miró
  • Josep Egozcue
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 444)

Abstract

Fluorescent in situ hybridization using three chromosome-specific centromeric human DNA probes was used to analyze the aneuploidy frequency in human-hamster two-cell embryos. With these techniques first mitotic division errors due to the effects of physical or chemical agents on human spermatozoa, such as non-disjunction and anaphase lag, can be easily detected. In control samples the estimated frequency of non-disjunction and anaphase lag was 3.4%. This assay can also detect premeiotic or meiotic errors. We estimated the same frequency (3.4%) for disomy, whereas monosomy was not found.

Keywords

Human Serum Albumin Human Spermatozoon Calcium Ionophore A23187 Pregnant Mare Serum Gonadotrophin Centromeric Probe 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Immaculada Ponsa
    • 1
  • Laura Tusell
    • 1
  • Ricard Álvarez
    • 1
  • Anna Genescà
    • 1
  • Rosa Miró
    • 1
  • Josep Egozcue
    • 1
  1. 1.Departament de Biologia Cellular i Fisiologia., Unitat de Biologia, Facultat de MedicinaUniversitat Autónoma de BarcelonaBellaterraSpain

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