Biochemical Characterization of Benomyl Inhibition on Endometrial Growth During Decidualization in Rats

  • Fitzgerald Spencer
  • Limen Chi
  • Ming-Xia Zhu
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 444)

Abstract

The antimitotic action of the systemic benzimidazole carbamate compound, benomyl, the basis for its fungitoxicity, was assessed in a mammalian system by selected biochemical endpoints of endometrial proliferation during decidualization in rats. The deciduoma, artificially induced on Day 4 of pseudopregnancy (PG), represents the maternal portion of the placenta that attains maximal growth between Days 9–11 PG. Deciduoma induction by surgical uterine trauma normally prolongs PG into the decidualization process. Measured endometrial parameters were the wet weight, protein for hypertrophy, DNA indicative of hyperplasia; enzymatic biomarkers- isocitrate dehydrogenase (ICDH) and the matrix metalloproteinases (MMPs); and serum progesterone which hormonally maintains decidual growth. Benomyl was administered by oral gavage in daily doses (500 mg/kg/rat in corn oil for 5 days, PG Days 5–9) and animals were sacrificed on PG Day 10. Benomyl caused significant reduction (P< 0.001) in endometrial wet weight, protein and DNA concentrations. ICDH activity was also significantly reduced (P< 0.01) following benomyl treatment. Of the two MMP species (72 and 92 kDa), whereas the 72 kDa was only slightly affected, the 92 kDa MMP was suppressed 2–3 fold by benomyl. Benomyl was without effect on the progesterone concentration. The findings suggest that during decidualization in rats, the anti-deciduogenic, antimitotic action of post-traumal benomyl treatment which occurred via the biochemical molecules (protein, DNA, ICDH and the MMPs) apparently was not mediated by progesterone.

Keywords

Endometrial Stromal Cell Methyl Benzimidazole Antimitotic Action Endometrial Proliferation ICDH Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1998

Authors and Affiliations

  • Fitzgerald Spencer
    • 1
    • 2
  • Limen Chi
    • 1
  • Ming-Xia Zhu
    • 1
  1. 1.Health Research CenterSouthern UniversityBaton RougeUSA
  2. 2.Biology DepartmentSouthern UniversityBaton RougeUSA

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