Binding of Daunorubicin and Doxorubicin to Erythrocytes Treated with Glutaraldehyde

  • Fazoil I. Ataullakhanov
  • Elena V. Kulikova
  • Victor M. Vitvitsky

Abstract

Intact human erythrocytes can bind anthracycline antibiotics (daunorubicin and doxorubicin) during incubation in the isotonic medium containing the antibiotic. Bound antibiotics can be immobilized in the erythrocytes using glutaraldehyde treatment. It was found, however, that erythrocytes pretreated with glutaraldehyde can uptake daunorubicin and doxorubicin from the incubation medium and do it faster than untreated cells. The antibiotics are readily released from these erythrocytes in the antibiotic-free medium. Our findings provide evidence that immobilization of anthracycline antibiotics in the erythrocytes using glutaraldehyde treatment is connected with the chemical binding of the antibiotics to different erythrocyte components.

Keywords

Incubation Medium Human Erythrocyte Cytosine Arabinoside Anthracycline Antibiotic Isotonic Medium 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Ataullakhanov, F.I, Batasheva, T.V., Bukhman, V.M., Komarova, S.V., Oreshkina, T.D. and Vitvitsky, V.M. [1994] Treatment of Rausher virus induced murine erythroblastic leukemia with rubomycin loaded erythrocytes, in Carrier and Bioreactor Red Blood Cells for Drug Delivery and Targeting (DeLoach, J.R. and Way, J.L. eds.)., pp. 177–183. Elsevier Science Ltd, Oxford.Google Scholar
  2. 2.
    Ataullakhanov, F.I., Batasheva, T.V. and Vitvitsky, V.M. [1994] Influence of temperature, daunorubicin concentration and suspension hematocrit on daunorubicin binding by human erythrocytes. Antibiotiki i Chimioterapiya 39, No. 9/10, 26-29 (in Russian).Google Scholar
  3. 3.
    Ataullakhanov, F.L, Batasheva, T.V., Vitvitsky, V.M. and Komarova, S.V. [1993] Effect of glutaraldehyde treatment on rubomycin and hemoglobin leakage from murine erythrocytes loaded with rubomycin. Biotekhnologiya No. 2, 40-43 (in Russian).Google Scholar
  4. 4.
    Ataullakhanov, F.I., Kulikova, E.V. and Vitvitsky, V.M. [1994] Doxorubicin binding by human erythrocytes, in Carrier and Bioreactor Red Blood Cells for Drug Delivery and Targeting (DeLoach, J.R. and Way, J.L. eds.)., pp. 163–168. Elsevier Science Ltd, Oxford.Google Scholar
  5. 5.
    Ataullakhanov, F.L, Vitvitsky, V.M., Kovaleva, V.L. and Mironova, S.B. [1992] Rubomycin loaded erythrocytes in the treatment of mouse tumor P388, in The Use of Resealed Erythrocytes as Carriers and Bioreactors (Magnani, M. and DeLoach, J.R. eds.)., pp. 209–213. Plenum Press, New York.CrossRefGoogle Scholar
  6. 6.
    Avrameas, S. and Ternynck, T. [1969] The cross-linking of proteins with glutaraldehyde and its use for the preparation of immunosorbents. Immunochemistry 6, 53–66.PubMedCrossRefGoogle Scholar
  7. 7.
    Cassiletti, P.A. and Katz, M.E. [1977] Chemotherapy for adult acute non lymphocytic leukemia with daunorubicin and cytosine arabinoside. Cancer Treat. Rep. 61, 1441–1445.Google Scholar
  8. 8.
    DeFlora, A., Benatti, U., Guida, L. and Zocchi, E. [1986] Encapsulation of doxorubicin in human erythrocytes. Proc. Natl. Acad. Sci. USA. 83, 7029–7033.CrossRefGoogle Scholar
  9. 9.
    Gaudreault, R.C., Bellemare, B. and Lacroix, J. [1989] Erythrocyte membrane-bound daunorubicin as a delivery system in anticancer treatment. Anticancer Res. 9, 1201–1205.PubMedGoogle Scholar
  10. 10.
    Kitao, T. and Hattori, K. [1980] Erythrocyte entrapment of daunomycin by amphotericin B without hemolysis. Cancer Res. 40, 1351–1353.PubMedGoogle Scholar
  11. 11.
    Kitao, T., Hattori, K. and Takeshita, M. [1978] Agglutination of leukemic cells and daunomycin entrapped erythrocytes with lectin in vitro and in vivo. Experientia 34, 94–95.PubMedCrossRefGoogle Scholar
  12. 12.
    Lefrak, E.A., Pitha, J., Rosenheim, S. and Gottlieb, J.A. [1973] A clinicopathologic analysis of adriamycin cardiotoxicity. Cancer 32, 302–314.PubMedCrossRefGoogle Scholar
  13. 13.
    Matherne, CM., Satterfield, W.C., Gasparini, A., Tonetti, M., Astroff, A.B., Schmidt, R.D., Rowe, L.D. and DeLoach, J.R. [1994] Clinical efficacy and toxicity of doxorubicin encapsulated in glutaraldehydetreated erythrocytes administered to dogs with lymphosarcoma. Am. J. Vet. Res. 55, 847–853.PubMedGoogle Scholar
  14. 14.
    Rachman, A., Fumagalli, A., Barbieri, B., Schein, P. and Casazza, A.M. [1986] Antitumor and toxicity evaluation of free doxorubicin and doxorubicin entrapped in cardiolipin liposomes. Cancer Chemother. Pharmacol. 16, 22–27.Google Scholar
  15. 15.
    Stenberg, C.P., Civin, C, Krischer, J., Culbert, S., Ragab, A., Ruymann, F.B., Ravindranath, J., Leventhal, B., Wilkinson, R. and Vietti, T.J. [1991] A comparison of induction and maintenance therapy for acute nonlymphocytic leukemia in childhood: results of a pediatric oncology group study. J. Clin. Oncol. 9, 247–288.Google Scholar
  16. 16.
    Tonetti, M., Astroff, B., Satterfield, W., DeFlora, A., Benatti, U. and DeLoach, J.R. [1990] Construction and characterization of adriamycin-loaded canine red blood cells as a potential slow delivery system. Biotechnol. Appl. Biochem. 12, 621–629.PubMedGoogle Scholar
  17. 17.
    Tonetti, M., Zocchi, E., Guida, Z., Polvani, C, Benatti, U., Biassoni, P., Romei, F., Guglielmi, A., Aschele, C. Sobrero, A. and DeFlora, A. [1992] Use of glutaraldehyde treated autologous human erythrocytes for hepatic targeting of doxorubicin, in The Use of Resealed Erythrocytes as Carriers and Bioreactors (Magnani, M. and DeLoach, J.R. eds.)., pp. 307–317. Plenum Press, New York.CrossRefGoogle Scholar
  18. 18.
    Zocchi, E., Tonetti, M., Polvani, C, Guida, Z., Benatti, U. and DeFlora, A. [1988] In vivo liver and lung targeting of adriamycin encapsulated in glutaraldehyde-treated murine erythrocytes. Biotechnol. Appl. Biochem. 10, 555–562.PubMedGoogle Scholar
  19. 19.
    Zocchi, E., Tonetti, M., Polvani, C., Guida, Z., Benatti, U. and DeFlora, A. [1989] Encapsulation of doxorubicin in liver-targeted erythrocytes increases the therapeutic index of the drug in murine metastatic model. Proc. Natl. Acad. Sci. USA. 86, 2040–2044.PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Fazoil I. Ataullakhanov
    • 1
  • Elena V. Kulikova
    • 1
  • Victor M. Vitvitsky
    • 1
  1. 1.Scientific Center for Hematology of RAMSMoscowRussia

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