Human Dendritic/Langerhans Cells Control Growth and Differentiation of CD40 Activated B Cells

  • Bertrand Dubois
  • Béatrice Vanbervliet
  • Jérôme Fayette
  • Catherine Massacrier
  • Francine Brière
  • Jacques Banchereau
  • Christophe Caux
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 417)

Abstract

During an immune response, dendritic cells (DC) capture the antigen at site of injury, and migrate through the afferent lymph stream to the lymph-nodes where they efficiently activate naive T cells. This T cell activation is followed by B cell recruitment, which occurs in the extrafollicular area, where DC home1. Accordingly, we wondered herein whether DC might directly interact with B cells, using DC generated in vitro from CD34+ progenitors, called Dendritic-Langerhans cells2,3 (D-Lc). As both DCs4 and B cells5 express functional CD40, we used CD40-ligand transfected L cells6 as surrogate activated T cells, to study the effect of DCs on B cell activation. We show that D-Lc enhanced the proliferation of CD40-activated naive B cells and Ig commited B lymphocytes (3–8 fold). In addition, D-Lc induced a 10 to 100 fold enhancement of IgG and IgA secretions by Ig committed B cells (essentially memory B cells). Most notably, in the presence of IL-2, D-Lc stimulated CD40-activated naive B cells to produce high amounts of IgM. Using transwells, we showed that the effect on B cell proliferation is soluble and independent of CD40 triggering on D-Lc. In contrast, the IgM production is partly dependent of soluble molecule(s) secreted after CD40 engagement on D-Lc. The use of anti-cytokine blocking antibodies indicate that D-Lc derived IL-12 is absolutely required (although not sufficient) for the differentiation of naive B cells into 1gM secreting cells in presence of IL-2.

Keywords

Dendritic Cell Germinal Center Cell Control Growth Fold Enhancement Umbilical Cord Blood Sample 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Bertrand Dubois
    • 1
  • Béatrice Vanbervliet
    • 1
  • Jérôme Fayette
    • 1
  • Catherine Massacrier
    • 1
  • Francine Brière
    • 1
  • Jacques Banchereau
    • 1
  • Christophe Caux
    • 1
  1. 1.Schering-PloughLaboratory for Immunological ResearchDardillyFrance

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