Constructing Artificial Antigen-Presenting Cells from Drosophila Cells

  • Jonathan Sprent
  • Zeling Cai
  • Anders Brunmark
  • Michael R. Jackson
  • Per A. Peterson
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 417)

Abstract

Stimulation of unprimed T cells is controlled by professional antigen-presenting cells (APC) such as dendritic cells (DC).1–3 The strong APC function of DC is presumed to reflect that these cells express a high density of major histocompatibility complex (MHC) molecules and a variety of costimulatory molecules. In this respect, activation of naive T cells by APC is thought to require two distinct signals: Signal t reflects T cell receptor (TCR) contact with specific peptides bound to MHC molecules, and Signal 2 is the consequence of other molecules on T cells, e.g. CD28, interacting with costimulatory molecules, e.g. B7 (B7-1, B7-2), on APC. Since many different accessory molecules on APC can express costimulatory function for T cells under defined conditions, which particular accessory molecules are essential for stimulating naive T cells is unclear. We have addressed this issue by constructing artificial APC from a Drosophila cell line by gene transfection.

Keywords

Major Histocompatibility Complex Major Histocompatibility Complex Class Proliferative Response Costimulatory Molecule Drosophila Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Jonathan Sprent
    • 1
  • Zeling Cai
    • 2
  • Anders Brunmark
    • 2
  • Michael R. Jackson
    • 2
  • Per A. Peterson
    • 2
  1. 1.Department of Immunology, IMM4The Scripps Research InstituteLa JollaUSA
  2. 2.R.W. Johnson Pharmaceutical Research InstituteSan DiegoUSA

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