Effects of Low Temperatures on Microorganisms, Plants, and Cold-Blooded Animals

  • M. J. Ashwood-Smith
Part of the The International Cryogenics Monograph Series book series (INCMS)

Abstract

Ecology can be defined as the science of the relationships between living organisms and their physical and biotic environment. The temperature of the environment is one of the major factors which has to be considered. As a science ecology is of great importance to man and his very existence is dependent on a knowledge of its laws. These laws were often obtained empirically as a result of hundreds of years of animal husbandry. Sometimes their workings were brought to light the hard way as has been witnessed recently with the widespread misuse of toxic insecticides. No organism lives without influencing another or in its turn being influenced by others. The numerous food chains in nature are pertinent examples of this maxim, although there are some primitive microorganisms which can obtain from inorganic sources all the necessary nutrients to enable them to live and reproduce. Bacteria, yeasts, and fungi are often regarded by laymen as enemies which cause diseases of man, animals, and plants. Some men will know, of course, that yeasts make life easier and happier by producing alcohol, that some fungi can be eaten and that others produce antibiotics. They will know also that sewage disposal is dependent on bacterial action and some may know that soil nitrogen is made available to plants by the action of bacteria. With these few examples, however, the ordinary man will be content and will leave the other and more delicate balances of nature to the scientists. No plants or animals evolve over the centuries in isolation from their surroundings. Any one of a number of small factors influencing any one part of the habitat will cause a change in the nature of the organisms being studied and in the rate and direction of evolution. Much of ecology is concerned with the complex relationships of microorganisms or animals and plants amongst themselves. The physical environment is also important and the subject matter of this chapter is in the last resort self defeating in ecological terms as it is neither possible nor perhaps correct to isolate for study one aspect of the physical environment, low temperature, in order to discuss in detail its implications in the life of animals, plants and microorganisms.

Keywords

Cold Acclimation Freezing Point Cold Shock None None Frost Resistance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Prosser, C. L., “Perspectives of Adaptation: Theoretical Aspects,” in Handbook of Physiology, Sect. 4, pp. 11–25, American Physiological Society, Washington, D.C., 1964.Google Scholar
  2. 2.
    Gressitt, J. L., Entomology of Antarctica, American Geophysical Union, Washington, D.C., Antarctic Research Series Vol. 10, NAS-NRC. Publication 1574, 1967.CrossRefGoogle Scholar
  3. 3.
    Swan, E. W., “The ecology of the High Himalayas,” Scient. Am. 205(4): 68 (1961).CrossRefGoogle Scholar
  4. 4.
    Meyer, G. H., Morrow, M. B., Wyss, O., Berg, T. E., and Littlepage, J. L., “Antarctica: the microbiology of an unfrozen saline pond,” Science (N.Y.), 138, 1103–1104 (1962).CrossRefGoogle Scholar
  5. 5.
    Clarke, K. U., “Insects and Temperature,” in (Rose, A. H., ed.) Thermobiology, pp. 293–352, Academic Press, London (1967).Google Scholar
  6. 6.
    Plough, H. H., “Spontaneous mutability in Drosophila,” Cold Spring Harb. Symp. Quant. Biol., 9, 127–137 (1941).CrossRefGoogle Scholar
  7. 7.
    Birkina, B. N., [“The effect of low temperature on the mutation process in Drosophila melanogaster,”] Biol. Zh., 7, 653–660 (1938).Google Scholar
  8. 8.
    Hampel, K. E. and Levan, A., “Breakage in human chromosomes induced by low temperature,” Hereditas, 51, 315–343 (1964).CrossRefGoogle Scholar
  9. 9.
    Shikama, K., “Effect of freezing and thawing on the stability of double helix of DNA,” Nature (London) 207, 529–530 (1965).CrossRefGoogle Scholar
  10. 10.
    Ashwood-Smith, M. J., “On the genetic stability of bacteria to freezing and thawing,” Cryobiology, 2, 39–43 (1965).CrossRefGoogle Scholar
  11. 11.
    Holm-Hansen, O., “Viability of blue-green algae after freezing,” Physiologia Pl., 16, 530–539 (1963).CrossRefGoogle Scholar
  12. 12.
    Bunt. J. S., “Thermal Energy as a Factor in the Biology of the Polar Regions,” in (Rose, A. H., ed.) Thermobiology, pp. 555–590, Academic Press, London (1967).Google Scholar
  13. 13.
    Smith, J. E., “A discussion on the terrestrial Antarctic ecosystem,” Phil. Trans. Roy. Soc., B, 252, 169–392 (1967).Google Scholar
  14. 14.
    Stanley, S. O. and Rose, A. H., “Bacteria and yeasts from lakes on Deception Island,” in A Discussion on the Terrestrial Antarctic Ecosystem, Phil. Trans. Roy. Soc., B, 252, 199-208 (1967).Google Scholar
  15. 15.
    Heal, O. W., Bailey, A. D., and Latter, P. M., “Bacteria, fungi, and protozoa in Signy Island soils compared with those from a temperate moorland,” in A Discussion on the Terrestrial Antarctic Ecosystem, Phil. Trans. Roy. Soc., B252, 191–198 (1967).CrossRefGoogle Scholar
  16. 16.
    Bunt, J. S. and Wood, E. J. F., “Microalgae and antarctic sea-ice,” Nature (London), 199, 1254–1255 (1963).CrossRefGoogle Scholar
  17. 17.
    Bunt, J. S., “Diatoms of antarctic sea-ice as agents of primary production,” Nature (London), 199, 1255–1257 (1963).CrossRefGoogle Scholar
  18. 18.
    Burkholder, P. R. and Mandelli, E. F., “Productivity of microalgae in Antarctic sea-ice,” Science (N.Y.), 149, 872–874 (1965).CrossRefGoogle Scholar
  19. 19.
    Mazur, P., “Physical and Chemical Basis of Injury in Single-celled Microorganisms Subjected to Freezing and Thawing,” in (Meryman, H. T., ed.) Cryobiology, pp. 213–315, Academic Press, London (1966).Google Scholar
  20. 20.
    Farrell, J. and Rose, A. H., “Low-temperature microbiology,” Adv. Appl. Microbiol., 7, 335–378 (1965).CrossRefGoogle Scholar
  21. 21.
    Proom, H., “The effect of cold on microorganisms: problems of freeze-drying,” Proc. Soc. Appl. Bact., 14, 261–268 (1951).CrossRefGoogle Scholar
  22. 22.
    Sherman, J. M. and Albus, W. R., “Physiological youth in bacteria,” J. Bact., 8, 127–139 (1923).Google Scholar
  23. 23.
    Farrell, J. and Rose, A. H., “Temperature Effects on Microorganisms,” in (Rose, A. H., ed.) Thermobiology, pp. 147–218, Academic Press, London (1967).Google Scholar
  24. 24.
    Ashwood-Smith, M. J., Bridges, B. A., and Munson, R. J., “Ultraviolet damage to bacteria and bacteriophage at low temperatures,” Science (N. Y.), 149, 1103–1105 (1965).CrossRefGoogle Scholar
  25. 25.
    Ashwood-Smith, M. J. and Bridges, B. A., “On the sensitivity of frozen microorganisms to ultraviolet radiation,” Proc. Roy. Soc. B, 168, 194–202 (1967).CrossRefGoogle Scholar
  26. 26.
    Bridges, B. A., Ashwood-Smith, M. J., and Munson, R. J., “On the nature of the lethal and mutagenic action of ultraviolet light on frozen bacteria,” Proc. Roy. Soc. B, 168, 203–215 (1967).CrossRefGoogle Scholar
  27. 27.
    Ashwood-Smith, M. J., Copeland, J., and Wilcockson, J., “Response of bacterial spores and Micrococcus radiodurans to ultraviolet irradiation at low temperatures,” Nature (London), 217, 337–338 (1968).CrossRefGoogle Scholar
  28. 28.
    Ashwood-Smith, M. J. and Bridges, B. A., “Ultraviolet mutagenesis in Escherichia coli at low temperatures,” Mutation Res., 3, 135–144 (1966).CrossRefGoogle Scholar
  29. 29.
    Smith, K. C. and O’Leary, M. E., “Photoinduced DNA-protein cross-links and bacterial killing: a correlation at low temperatures,” Science (N.Y.), 155, 1024–1026 (1967).CrossRefGoogle Scholar
  30. 30.
    Donnellan, J. E., Hosszu, J. L., Rahn, R. O., and Stafford, R. S., “Effect of temperature on the photobiology and photochemistry of bacterial spores,” Nature (London), 219, 964–965 (1968).CrossRefGoogle Scholar
  31. 31.
    Downes, A. and Blunt, T. P., “Researches on the effect of light upon Bacteria and other organisms,” Proc. Roy. Soc., 26, 488–500 (1877).CrossRefGoogle Scholar
  32. 32.
    Appleyard, G. J., “The photosensitivity of Semliki Forest and other viruses,” J. Gen. Virol., 1, 143–152 (1967).CrossRefGoogle Scholar
  33. 33.
    Ashwood-Smith, M. J., Copeland, J., and Wilcockson, J., “Sunlight and frozen bacteria,” Nature (London), 214, 33–35 (1967).CrossRefGoogle Scholar
  34. 34.
    Bruch, C.W., “Microbes in the upper atmosphere and beyond,” Symp. Soc. Gen. Microbiol., 17, 345–374 (1967).Google Scholar
  35. 35.
    Pady, S. M. and Kelly, C. D., “Aerobiological studies of fungi and bacteria over the Atlantic Ocean,” Can. J. Bot., 32, 202–212 (1954).CrossRefGoogle Scholar
  36. 36.
    Hollaender, A., Swanson, C. P., and Posner, I., “The sun as a source of mutation-producing radiation,” (Abstr.), Am. J. Bot., 33, 830 (1946).Google Scholar
  37. 37.
    Siegel, S. M., Nathan, H. C., and Roberts, K., “Experimental biology of ammonia-rich environments: optical and isotopical evidence for vital activity in Penicillium in liquid ammonia-glycerol media at − 40°C,” Proc. Natn. Acad. Sci. (U.S.A.), 60, 505–508 (1968).CrossRefGoogle Scholar
  38. 38.
    Levitt, J., “Winter Hardiness in Plants,” in (Meryman, H. T., ed.) Cryobiology, pp. 495–563, Academic Press, London (1966).Google Scholar
  39. 39.
    Levitt, J., “Cryobiology as viewed by the botanist,” Cryobiology, 1, 11–17 (1964).CrossRefGoogle Scholar
  40. 40.
    Krasavtsev, O. A., “Frost Hardening of Woody Plants at Temperatures below Zero,” in Cellular Injury and Resistance in Freezing Organisms, pp. 131–141, Hokkaido University, Institute of Low-Temperature Science, Japan (1967).Google Scholar
  41. 41.
    Sakai, A., “Survival of Plant Tissue at Super-low Temperatures by Rapid Cooling and Rewarming,” in Cellular Injury and Resistance in Freezing Organisms, pp. 119–130, Hokkaido University, Institute of Low-Temperature Science, Japan (1967).Google Scholar
  42. 42.
    Siminovitch, D., Gfeller, F., and Rheaume, B., “The Multiple Character of the Biochemical Mechanism of Freezing Resistance of Plant Cells,” in Cellular Injury and Resistance in Freezing Organisms, pp. 93–117, Hokkaido University, Institute of Low-Temperature Science, Japan (1967).Google Scholar
  43. 43.
    Li, P. H. and Weiser, C. J., “Metabolism of nucleic acids in apple twig during cold acclimation (Abstr.),” Cryobiology, 4, 275 (1968).Google Scholar
  44. 44.
    Weiser, C. J., “Endogenous control of cold acclimation in woody plants (Abstr.),” Cryobiology, 4, 277 (1968).Google Scholar
  45. 45.
    Heber, U. and Ernest, R., “A Biochemical Approach to the Problem of Frost Injury and Frost Hardiness,” in Cellular Injury and Resistance in Freezing Organisms, pp. 63–77, Hokkaido University, Institute of Low-Temperature Science, Japan (1967).Google Scholar
  46. 46.
    Kanwisher, J. W., “Freezing in Intertidal Animals,” in (Meryman, H. T., ed.), Cryobiology, pp. 487–494, Academic Press, London (1966).Google Scholar
  47. 47.
    Kanwisher, J. W., “Freezing in Intertidal Animals,” Biol. Bull., 109, 56–63 (1955).CrossRefGoogle Scholar
  48. 48.
    Williams, R. J., “The mechanism of cryoprotection in the intertidal mollusc Mytilus (Abstr.),” Cryobiology, 4, 250–251 (1968).Google Scholar
  49. 49.
    Williams, R. J., “Studies on the freezing resistance of intertidal molluscs,” Cryobiology, 2, 299–300 (1966).Google Scholar
  50. 50.
    Siminovitch, D. and Briggs, D. R., “The chemistry of the living bark of the black locust tree in relation to frost hardiness. I Seasonal variation in protein content,” Arch. Biochem., 23, 8–17 (1949).Google Scholar
  51. 51.
    Smith, A. U., “The Effects of Subzero Temperatures on Poikilothermic Animals,” in Biological Effects of Freezing and Thawing, pp. 270–303, Edward Arnold, London (1961).Google Scholar
  52. 52.
    Scholander, P. F., Dam, L. van, Kanwisher, J. W., Hammel, H. T., and Gordon, M. S., “Supercooling and osmoregulation in Arctic fish,” J. Cell. Comp. Physiol., 49, 5–24 (1957).CrossRefGoogle Scholar
  53. 53.
    Downes, J. A., “Adaptations of insects in the Arctic,” A. Rev. Ent., 10, 257–274 (1965).CrossRefGoogle Scholar
  54. 54.
    Asahina, E., “Freezing and Frost Resistance in Insects,” in (Meryman, H. T., ed.), Cryobiology, pp. 451–486, Academic Press, London (1966).Google Scholar
  55. 55.
    Salt, R. W., “Principles of insect cold-hardiness,” A. Rev. Ent., 6, 55–74 (1961).CrossRefGoogle Scholar
  56. 56.
    Salt, R. W., “Terrestrial Animals in Cold: Arthropods,” in Handbook of Physiology, Sect. 4, pp. 349–355, American Physiological Society, Washington, D.C. (1964).Google Scholar
  57. 57.
    Payne, N. M., “Freezing and survival of insects at low temperatures,” J. Morph., 43, 521–546 (1927).CrossRefGoogle Scholar
  58. 58.
    Hinton, H. E., “Cryptobiosis in the larva of Polypedilum vanderplanki Hint. (Chironimidae),” J. Insect Physiol., 5, 286–300 (1960).CrossRefGoogle Scholar
  59. 59.
    Wyatt, G. R. and Kalf, G. F., “Organic components of insect hemolymph,” in Proceedings of the 10th International Congress of Entomology, Vol. 2, p. 333 (Abstr.) (1958).Google Scholar
  60. 60.
    Salt, R. W., “Natural occurrence of glycerol in insects and its relation to their ability to survive freezing,” Can. Ent., 89, 491–494 (1957).CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1970

Authors and Affiliations

  • M. J. Ashwood-Smith
    • 1
  1. 1.Radiobiological Research UnitMedical Research CouncilHarwell, DidcotEngland

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