Applicability and Limitations of Three-Dimensional NMR Spectroscopy for the Study of Proteins in Solution

  • Rolf Boelens
  • Christian Griesinger
  • Lewis E. Kay
  • Dominique Marion
  • Erik R. P. Zuiderweg
Chapter
Part of the NATO ASI Series book series (NSSA, volume 225)

Abstract

Assignment procedures for backbone resonances of proteins are outlined for homonuclear and heteronuclear three-dimensional NMR spectroscopy. Phasecycling procedures in fast 3D experiments are discussed. Examples of data space reducing processing techniques are given. A new triple resonance based assignment procedure for protein resonances is outlined. The general advantages and disadvantages of homonuclear and heteronuclear three-dimensional NMR spectroscopy are discussed.

Keywords

Cross Peak Assignment Procedure Sequential Assignment Phase Cycling HNCO Spectrum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1991

Authors and Affiliations

  • Rolf Boelens
    • 1
  • Christian Griesinger
    • 2
  • Lewis E. Kay
    • 3
  • Dominique Marion
    • 4
  • Erik R. P. Zuiderweg
    • 5
  1. 1.Department of ChemistryUniversity of UtrechtCH UtrechtThe Netherlands
  2. 2.Institute for Organic ChemistryJohann Wolfgang Goethe UniversityFrankfurt-am-Main 50Federal Republic of Germany
  3. 3.NIDDK, National Institutes of HealthLaboratory of Chemical PhysicsBethesdaUSA
  4. 4.Centre de Biophysique MoleculaireC.N.R.S.Orleans Cedex 2France
  5. 5.Pharmaceutical DiscoveryAbbott LaboratoriesAbbott ParkUSA

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