Track Structure, Chromosome Geometry and Chromosome Aberrations

  • David J. Brenner
  • John F. Ward
  • Rainer K. Sachs
Part of the Basic Life Sciences book series (BLSC, volume 63)

Abstract

The joint role of radiation track structure and chromosome geometry in determining yields of chromosome aberrations is discussed. Ideally, the geometric models of chromosomes used for analyzing aberration yields should have the same degree of realism as track structure models. However, observed chromosome aberrations are produced by processes on comparatively large scales, e.g., misrepair involving two DSB located on different chromosomes or two DSB separated by millions of base pairs on one chromosome, and quantitative models for chromatin on such large scales have to date almost never been attempted. We survey some recent data on large-scale chromosome geometry, mainly results obtained with fluorescence in situ hybridization (“chromosome painting”) techniques. Using two chromosome models suggested by the data, we interpret the relative yields, at low and high LET, of inter-chromosomal aberrations compared to intra-chromosomal, inter-arm aberrations. The models consider each chromosome confined within its own “chromosome localization sphere,” either as a random cloud of points in one model or as a confined Gaussian polymer in the other. In agreement with other approaches, our results indicate that at any given time during the G 0/G l part of the cell cycle a chromosome is largely confined to a sub-volume comprising less than 10% of the volume of the cell nucleus. The possible significance of the ratio of inter-chromosomal aberrations to intra-chromosomal, inter-arm aberrations as an indicator of previous exposure to high LET radiation is outlined.

Keywords

Autocorrelation Function Chromosomal Aberration Double Strand Break Ionization Cluster Chromosome Aberration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • David J. Brenner
    • 1
  • John F. Ward
    • 2
  • Rainer K. Sachs
    • 3
  1. 1.Center for Radiological ResearchColumbia UniversityNew YorkUSA
  2. 2.Department of RadiologyUniversity of California at San DiegoLa JollaUSA
  3. 3.Department of Physics and Department of MathematicsUniversity of CaliforniaBerkeleyUSA

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