Biophysical Aspects of GABA- and Glycine-Gated Cl− Channels in Mouse Cultured Spinal Neurons
γ-Aminobutyric acid (GABA) and glycine are the main inhibitory transmitter substances in the mammalian CNS (Krnjević and Schwartz, 1967; Werman et al., 1967). Following their release from presynaptic nerve terminals, GABA and glycine molecules bind to postsynaptic receptors and cause an increase in membrane conductance to chloride ions (Barker and Ransom, 1978). The molecular components involved in the Cl− conductance increase, the GABA receptor (GABAR) and glycine receptor (GlyR) channels, have been studied electrophysiologically by using patch-clamp current-recording techniques (Sakmann et al., 1983; Hamill et al., 1983; Bormann et al., 1987). The results obtained from mouse cultured spinal neurons are presented below in order to illustrate the conductance and gating properties of the two receptor-regulated membrane channels.
KeywordsReceptor Channel Glycine Receptor Spinal Neuron Membrane Channel Presynaptic Nerve Terminal
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