Further Characterization of DPP IV-β, a Novel Cell Surface Expressed Protein with Dipeptidyl Peptidase Activity

  • Julià Blanco
  • Etienne Jacotot
  • Christian Callebaut
  • Bernard Krust
  • Ara G. Hovanessian
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 421)

Abstract

By using a CD26 negative human lymphoblastoid cell line (C8166), here we describe the characterization of a cell-surface protein which manifests CD26-like dipeptidyl peptidase IV (DPP IV) activity. This protein, referred to as DPP IV-β, shows a higher KM value for Gly-Pro-pNA than CD26 (0,31 mM compared to 0,11 mM, respectively). In addition, DPP IV-β was found not to bind 125I-labeled adenosine deaminase (a property of human CD26). Gel filtration experiments using extracts from C8166 and MOLT4 (a CD26 positive human T cell line) cells, revealed that the apparent molecular mass of DPP IV-β is 82 kDa, whereas that of CD26 is 110 kDa. In order to conveniently differentiate both activities, a new family of inhibitors, that selectively blocks peptidase activity associated to CD26, has been developed.

Keywords

C8166 Cell Adenosine Deaminase Dipeptidyl Peptidase MOLT4 Cell DPPIV Inhibitor 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Alba, F. et al (1995) Peptide.+, 16: 325–329CrossRefGoogle Scholar
  2. 2.
    Smyth, M. and Cuinn G.O. (1994) J of Neurochem.; 63: 1439–1445CrossRefGoogle Scholar
  3. 3.
    Morrison, M.E. et al (1993) J. of Exp. Med.; 177: 1135–1143CrossRefGoogle Scholar
  4. 4.
    Kameoka, J. et al (1993) Science; 261: 166–469CrossRefGoogle Scholar
  5. 5.
    De Meester, I. et al (1994) Eur. J. oflmmunol.; 24: 566–570CrossRefGoogle Scholar
  6. 6.
    Darmoul, D. (1992) J. Biol. Chem.; 267: 4824–4833Google Scholar
  7. 7.
    Tanaka, T. (1992) J. Immunol.; 149: 481–486PubMedGoogle Scholar
  8. 8.
    Marguet, D. et al (1992) J. Biol. Chem.; 267: 2200–2208PubMedGoogle Scholar
  9. 9.
    Ogata, S. et al (1992) Biochemistry; 31: 2582–2587PubMedCrossRefGoogle Scholar
  10. 10.
    Roberts, C.J. et al (1992) J. Cell. Biol.; 119: 69–83PubMedCrossRefGoogle Scholar
  11. 11.
    Fleischer, B. (1994) Immunol. Today; 15: 180–184PubMedCrossRefGoogle Scholar
  12. 12.
    Bohm, S.K. et al (1995), Biochem. J.; 311: 835–843PubMedGoogle Scholar
  13. Torimoto, Y. et al (991) J. Immunol.; 147: 2514–2517Google Scholar
  14. 14.
    Dang, N.H. et al (1990) J. Immunol.; 145: 3963–3971PubMedGoogle Scholar
  15. 15.
    Martin, M. et al (1995) J. Immunol.; 155: 4630–4643PubMedGoogle Scholar
  16. 16.
    Callebaut, C. et al (1993) Science; 262: 2045–2050PubMedCrossRefGoogle Scholar
  17. 17.
    Morimoto, C. et al (1994)Proc. Natl. Acad. Sci. USA; 91: 9960–9964Google Scholar
  18. 18.
    Gutheil, W.G. et al (1994) Proc. Natl. Acad. Sci. USA; 91: 6594–6598PubMedCrossRefGoogle Scholar
  19. 19.
    Blanco, J. et al. (1996) Exp. Cell Res.; 225: 102–111PubMedCrossRefGoogle Scholar
  20. 20.
    Duke-Cohan, J. S., et al (1995) J. Biol. Chem.: 270: 14107–14114PubMedCrossRefGoogle Scholar
  21. 21.
    Duke-Cohan, J.S. et al (1996) J. Immunol.; 156: 1714–1721PubMedGoogle Scholar
  22. 22.
    Jacotot, E. et al (1996) Eue. J. Biochem., 239: 248–258CrossRefGoogle Scholar
  23. 23.
    Werner, A. et al (1994) AIDS; 9: 1348–1349CrossRefGoogle Scholar
  24. Schön, E. et al (1991 1 Brol. Chem. Hoppe Seyler; 372: 305–311Google Scholar
  25. 25.
    Born, J. (1994) Eue J. Cell. Biol_;Supl. 40: 23Google Scholar
  26. 26.
    Rahfeld, J. et al (1991) Biol. Chem. Hoppe-Seyler; 372: 313–318PubMedCrossRefGoogle Scholar
  27. 27.
    Cole, S.R. et al (1987) Mol. Immunol.; 24: 699–705PubMedCrossRefGoogle Scholar
  28. 28.
    Scanlan, M.J. (1994) Proc. Natl. Acad. Sci. USA; 91: 5657–5661PubMedCrossRefGoogle Scholar
  29. 29.
    Rettig, W.J. et al (1994) Int. J. Cancer; 58: 385–392PubMedCrossRefGoogle Scholar
  30. 30.
    Yaron, A. and Naider, F. (1993) Critical Rev. in Biochem. and Mol. Biol.; 28: 31–81CrossRefGoogle Scholar
  31. 31.
    Kieffer, T. et al (1995) Endocrinol.; 136: 3585–3596CrossRefGoogle Scholar
  32. 32.
    Oravezc, T. et al (1996)1. Immunol.; 157: 1329–1332Google Scholar

Copyright information

© Springer Science+Business Media New York 1997

Authors and Affiliations

  • Julià Blanco
    • 1
  • Etienne Jacotot
    • 1
  • Christian Callebaut
    • 1
  • Bernard Krust
    • 1
  • Ara G. Hovanessian
    • 1
  1. 1.Unité de Virologie et Immunologie Cellulaire UA CNRS 1157Institut PasteurFrance

Personalised recommendations