Co-Incubation of Lymphocytes with Fibroblast-Like Synoviocytes and other Cell Types Can Induce Lymphocytic Surface Expression of Aminopeptidase N/CD13
Aminopeptidase N (APN, EC 126.96.36.199) is an ubiquitously occurring transmembrane ectoenzyme. Sequence comparisons of the cloned cDNA showed that APN is identical to CD13 antigen1. The coding part of the CD13 gene is encoded by 20 exons2. CD13 has been considered to be a myeloid-specific marker because lymphocytes of peripheral blood and tonsills always are CD13 negative. However, we have previously demonstrated the expression of CD l3 on synovial T cells from patients with different forms of arthritis3 or on tumor-infiltrating lymphocytes from renal cell carcinoma4. To learn more about conditions in tissues resulting in the expression of CD13 on lymphocytes, we incubated tonsillar T and B cells as well as peripheral blood lympho-cytes (PBL) with various mediators in the absence and presence of different stromal cells.
KeywordsCD13 mRNA Human Renal Cell Carcinoma Human Renal Cell Carcinoma Cell Kidney Tubular Epithelial Cells11 Natural Killer Cell Surface
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- 2.Lerche C, Vogel LK, Shapiro LH, Noren O. Sjöström H (1996) Mamm Genome 712–713.Google Scholar
- 3.Riemann D, Schwachula A, Hentschel M, Langner J (1993) Demonstration of CD13iaminopeptidase N on synovial fluid T cells from patients with different forms of joint effusions. Immunobiol 187: 24–35.Google Scholar
- 6.Scott S, Pandolfi F, Kurnick T (1990) Fibroblasts mediate T cell survival: A proposed mechanism for retention of primed cells. J Exp Med 172: 1873–1876.Google Scholar
- 8.Riemann D, Kehlen A, Thiele K, Löhn M, Langner J (1997) Induction of aminopeptidase N/CD13 on human lymphocytes upon adhesion to fibroblast-like synoviocytes, endothelial cells, epithelial cells and monocytes/macrophages. 1 Immunol in press.Google Scholar
- 10.Riemann D. Wollert H-G, Menschikowski J. Mittenzwei S, Langner.1 (1994) Immunophenotype of lymphocytes in pericardial fluid from patients with different forms of heart disease. Int Arch Allerg Immunol 104: 48–56.Google Scholar
- 11.Riemann D, Kehlen A, Langner J (1995) Stimulation of the expression and the enzyme activity of aminopeptidase N/CDI3 and dipeptidylpeptidase IV/CD26 on human renal cell carcinoma cells and renal tubular epithelial cells by T cell-derived cytokines, such as IL-4 and IL-13. Clin Exp. Immun 100: 277–283.Google Scholar
- 12.Wex T, Lendeckel U, Wex H, Frank K, Ansorge S (1995) Quantification of aminopeptidase N mRNA in T cells by competitive PCR. FIBS Lett 374: 341–344.Google Scholar
- I3. Wex T, Lendeckel U, Reinhold D, Kühne T, Arndt M, Frank K, Ansorge S (1996) Antisense-mediated inhibition of aminopeptidase N (CD13) markedly decreases growth rates of hematopoictic tumour cells. Chapter in this book.Google Scholar
- 14.Sanderink GJ, Artur Y, Siest G (1988) Human aminopeptidases. A review of the literature. J Clin Chem Clin Biochem 26: 795–807.Google Scholar
- I7. Menrad A, Speicher D, Walker J, Herlyn M (1993) Biochemical and functional characterization of aminopeptidase N expressed by human melanoma cells. Cancer Res 53: 1450–1455.Google Scholar
- 18.Saiki I, Fujii H, Yoneda H, Abe F, Nakajima M, Tsuruo T, Azuma I (1993) Role of aminopeptidase N (CD 13) in tumor-cell in vasion and extracellular matrix degradation. Int J Cancer 54: 137–143.Google Scholar
- 19.Larsen SL, Ostergaard L, Buus S, Stryhn A (1996) T cells responses affected by aminopeptidase N (CD13)mediated trimming of major histocompatibility complex class II - bound peptides. J I?xp Med 184: 183–189.Google Scholar