Abstracts of Posters

  • Robert Gregerson
  • Susan Miller
  • Scott Twary
  • Steven Gantt
  • Carroll Vance

Abstract

Glutamate synthase (NADH-GOGAT) is one of several proteins whose expression is either enhanced in, or specific to developing legume root nodules induced by (Brady)Rhizobium infection. This enzyme catalyzes the second committed step in the assimilation of symbiotically fixed nitrogen; the transamidation of 2-oxoglutarate by glutamine to yield two moles of glutamate. We have previously purifed alfalfa root nodule NADH-GOGAT and raised antibodies against this protein. To investigate the regulation of the gene encoding this enzyme, we have isolated a full length cDNA clone for NADH-GOGAT from an alfalfa nodule cDNA library. This 7.5 kb cDNA includes the sequence encoding the mature 210 kd protein (the amino terminus of which has been sequenced), as well as an apparent transit peptide. Northern blots indicate that mRNA levels are developmentally regulated in the nodule, showing a similar pattern to that of previously characterized genes encoding aspartate aminotransferase and phosphoenoylpyruvate carboxylase.

Keywords

Acyl Carrier Protein Inflorescence Meristem Auxin Transport Inhibitor Precocious Germination Globulin Gene 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Robert Gregerson
    • 1
    • 3
  • Susan Miller
    • 2
  • Scott Twary
    • 2
  • Steven Gantt
    • 3
  • Carroll Vance
    • 1
    • 3
  1. 1.Plant Science Research UnitUSDA-ARSUSA
  2. 2.Department of Agronomy and Plant GeneticsUniversity of MinnesotaSt. PaulUSA
  3. 3.Plant Biology DepartmentUniversity of MinnesotaSt. PaulUSA

Personalised recommendations