Transport Studies in Isolated Enterocytes
The purpose of this paper is to illustrate experimental strategies that may be adopted in the laboratory to approach transport studies in isolated enterocytes with isotopic methods. Since the work carried out with this biological preparation is vast I shall focus on methodologies set up to: a) investigate the energetics of organic solute active transport (e.g.sugars and dipeptides), b) reveal the separate pathways involved in the transfer of a given solute across the cell membrane (e.g. K+ transport) and c) study intracellular transport events.
KeywordsIntestinal Epithelial Cell Incubation Buffer Cotransport System Radiolabelled Substrate Membrane Vesicle Preparation
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