Identification of Selected Campylobacter Spp. Using the ARMS Technique

  • B. Allan
  • L. M. Steyn
  • Siobahn McCarthy
  • A. Lastovica
  • B. Gay Elisha

Abstract

The precise identification of unusual members of the genus Campylobacter is often problematic7. By and large, speciation is based on a few phenotypic tests that are not always reliable7. Alternative tools for identifying species are based on the genotype rather than on the phenotype of an organism. Genotypic methods have been used to discriminate the thermophilic Campylobacter species1,2. Recently, a simple PCR-based method, the Amplification Refractory Mutation System (ARMS), for the characterization of DNA polymorphisms was described5. Where species specific base differences can be identified, the authors suggest that the technique might prove useful in the speciation of bacteria. The work described here evaluates ARMS in the identification of C.mucosalis, C.concisus and C.curvus, species that are impossible to differentiate by phenotypic tests alone4.

Keywords

Universal Primer Phenotypic Test Amplification Refractory Mutation System Cific Primer Proof Reading Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Eyers M., Chapelle S., Van Camp G., Goossens H. and De Wachter R. (1993) J. Clin. Microbiol. 31, 3340–3343.PubMedGoogle Scholar
  2. 2.
    Giesendorf B., Van Belkum A., Koekman A., Stegman H., Henkens M., Van der Plas J., Goossens H., Niesters H. and Quint W. (1993) J. Clin. Microbiol. 31, 1541–1546.PubMedGoogle Scholar
  3. 3.
    Johnson J. (1990) J. Mol. Biol. 3, 208–218.Google Scholar
  4. 4.
    Lastovica A., Le Roux E., Warren R. and Klump H. (1994) J. Clin. Microbiol. 32, 2238–2239.Google Scholar
  5. 5.
    Newton C., Graham A., Heptinstall L., Powell S., Summers C., Kalsheker N., Smith J. and Markham A. (1989) Nuc.Acids Res. 17, 2503–2516.CrossRefGoogle Scholar
  6. 6.
    Rolfs A., Schuller L, Finckh U. and Weber-Rolfs I. (1992) In PCR: Clinical Diagnosis and Research, p. 34, Springer-Verlag, Berlin Heidelberg.CrossRefGoogle Scholar
  7. 7.
    Vandamme P. and Goossens H. (1992) Zentralblatt für Bakteriol. 276, 447–472.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • B. Allan
    • 1
  • L. M. Steyn
    • 1
    • 2
  • Siobahn McCarthy
    • 2
  • A. Lastovica
    • 3
  • B. Gay Elisha
    • 1
    • 2
  1. 1.Department of Medical MicrobiologyGroote Schuur HospitalCape TownSouth Africa
  2. 2.Medical SchoolUniversity of Cape TownObservatory, Cape TownSouth Africa
  3. 3.Department of Medical MicrobiologyRed Cross War Memorial Children’s HospitalRondebosch, Cape TownSouth Africa

Personalised recommendations