Development of a Molecular Engineered Vaccine for C. Botulinum Neurotoxins

  • H. F. LaPenotiere
  • M. A. Clayton
  • J. E. Brown
  • J. L. Middlebrook

Abstract

Recent work involving recombinant tetanus neurotoxin fragments demonstrate the “C fragment” (Hc domain) is a good vaccine candidate. This report describes initial efforts to determine if botulinum neurotoxin Hc fragments will also elicit aprotective immune response. Using clones encoding part of the heavy chain, gene segment constructs were designed to produce a native Hc polypeptide or an Hc polypeptide fused to E. coli maltose binding protein. Problems were encountered with both systems. Specifically, the construct for the native protein appeared to be unstable, while the fusion product appeared to be packaged in inclusion bodies that formed insoluble aggregates. Preliminary trials with animals indicated that vaccination with the fusion product conferred protection against native toxin challenge.

Keywords

Botulinum Toxin Fusion Product Maltose Binding Protein Botulinum Neurotoxin Tetanus Toxin 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • H. F. LaPenotiere
    • 1
  • M. A. Clayton
    • 1
  • J. E. Brown
    • 1
  • J. L. Middlebrook
    • 1
  1. 1.Toxinology Division USAMRIIDFort DetrickFrederickUSA

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