Long-Term Effects of Botulinum Type A Neurotoxin on the Release of Noradrenaline from PC12 Cells
Clostridium botulinum type A neurotoxin (BoNTA) slowly inhibits the calcium-dependent release of noradrenaline from PC12 cells in a dose-dependent manner. The effects of BoNTA on PC12 cells are shown to persist for several days and in subsequent cell generations. Examination of the molecular form of cell-associated 125I-labelled BoNTA after 96h incubation within PC12 cells showed virtually no detectable degradation of either the heavy or light chains of the neurotoxin. The data suggest that BoNTA is relatively stable within the PC12 cell with a half-life in the order of several days. More than 50% of the membrane-associated BoNTA within the cell exists in the form of high molecular weight, disulphide-linked aggregates and these appear to be enriched in the light chain of the neurotoxin. A possible role in action of BoNTA for these aggregates is discussed.
KeywordsPC12 Cell Light Chain Supernatant Fluid Noradrenaline Release Botulinum Neurotoxin
Unable to display preview. Download preview PDF.
- Shone, C.C., 1987, Clostridium botulinum neurotoxins: their and modes of action, in:“Natural Toxicants in Foods”, D. Watson ed., pp 11–51, Ellis Horwood Ltd., Chichester, U.K.Google Scholar