Inhibition of Norepinephrine Secretion from Digitonin Permeabilized PC12 Cells by C. Botulinum Type D Toxin

  • Noriko Yokosawa
  • Kouichi Kimura
  • Kayo Tsuzuki
  • Nobuhiro Fujii
  • Bunei Syuto
  • Keiji Oguma

Abstract

Toxigenic strains of Clostridium botulinum produced seven immunological distinct neurotoxins, type A-G. Neurotoxins are 150-kDa proteins, synthesized as single chain molecules and nicked to the dichain form by endogenous protease(s) in the bacterial culture. Dichain form consists of a heavy chain (approx. 100 kDa) and a light chain (approx. 50 kDa) held together by at least one disulfide bond and noncovalent bonds.

Keywords

PC12 Cell Botulinum Toxin Botulinum Neurotoxin Tetanus Toxin Clostridium Botulinum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Noriko Yokosawa
    • 1
  • Kouichi Kimura
    • 1
  • Kayo Tsuzuki
    • 1
  • Nobuhiro Fujii
    • 1
  • Bunei Syuto
    • 2
  • Keiji Oguma
    • 1
  1. 1.Department of MicrobiologySapporo Medical CollegeJapan
  2. 2.Department of Biochemistry Faculty of Veterinary MedicineHokkaido UniversitySapporo, 060Japan

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