Protein Purification by Selective Adsorption on Immobilized Pyridinium Ligands

  • That T. Ngo

Abstract

Avid AL is an affinity gel with synthetic low molecular weight affinity ligands capable of selectively purifying IgG from the serum of all animals tested. By packing the gel in a radial flow column, a high flow rate can be applied and 12 g of highly purified IgG can be obtained within 5 hours directly from the serum. Immunoglobulins bound to Avid AL can be eluted with 0.05 M sodium acetate at pH 3 or at pH 7.4 with a neutral buffer containing “electron-rich” compounds. The affinity gel appears to be suitable under a varieties of storage and depyrogenation conditions.

Keywords

Aromatic Amino Acid Protein Adsorption Selective Adsorption Charge Transfer Complex Aromatic Amino Acid Residue 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1994

Authors and Affiliations

  • That T. Ngo
    • 1
  1. 1.BioProbe International Inc.TustinUSA

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