Influence of Intracarotid Hyperthermic Saline Infusion on the Blood-Brain Barrier Permeability in Anesthetized Rats

  • Baria Öztaş
  • Mutlu Küçük
Part of the Advances in Behavioral Biology book series (ABBI, volume 46)

Summary

Retrograde infusion of hyperthermic saline solution at 43°C into the left external carotid artery increases cerebrovascular permeability to Evans-blue albumin in the left cerebral hemisphere in normothermic Wistar rats. Isotonic saline solutions at 37°C for Group I and at 43°C for Group II were infused for 30-s at a constant rate of 0.12 ml/s into the left external carotid artery. All animals receiving hyperthermic saline perfusion, had disturbed blood-brain barrier permeability. Based on visual inspection, disruption grade in the left hemispheres of 6 out of 10 animals were 3+. Mean values for Evans-blue dye were found to be 0.28±0.06 mg% in left hemisphere after normothermic saline infusion (Group I), and 2.4±0.5 mg% in the same hemisphere after hyperthermie saline infusion (Group II). The difference was found to be significant between Group I and Group II (P<0.01).

Keywords

Left Hemisphere Saline Infusion Left Internal Carotid Artery Solution Saline Body Weight Dose 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Résumé

La perfusion par voie rétrograde d’une solution saline à 43°C, dans la carotide externe gauche de rats normaux Wistar conduit à une augmentation de la perméabilité vasculaire cérébrale pour l’albumine/bleu Evans. Deux groupes de rats ont été perfusés pendant 30 s à un débit constant de 0.12 ml/s avec une solution saline isotonique à 37° C (rats temoins) ou à 43° C. Tous les animaux perfusés avec la solution à 43° C présentent des perturbations de la perméabilité de la BHE. Une observation visuelle a permis de déterminer que le degré de rupture de la BHE dans les hémisphères cérébraux gauches de 6 animaux sur 10 atteignait le niveau 3+.Le passage du bleu Evans est de 0.28 +/− 0.06 mg% dans l’hémisphère gauche des rats témoins et de 2.4 +/− 0.5 mg% dans l’hémisphère gauche des rats perfusés avec la solution hyperthermique. La différence entre les deux groupes est significative (p<0.01).

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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Baria Öztaş
    • 1
  • Mutlu Küçük
    • 1
  1. 1.Department of Physiology Istanbul Faculty of MedicineUniversity of IstanbulÇapa/IstanbulTurkey

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