Inhibition of GFAP Synthesis with Antisense Nucleic Acid Constructs

  • Albert C. H. Yu
  • Yuen Ling Lee
  • Lawrence F. Eng
Part of the Altschul Symposia Series book series (ALSS, volume 2)

Abstract

Glial fibrillary acidic protein (GFAP), the major component of the intermediate filament in differentiated astrocytes (Eng et al., 1971; Eng, 1985), is extensively synthesized within and adjacent to the site of injury (Eng, 1988a; Condorelli et al., 1990; Hozumi et al., 1990; Vijayan et al., 1990). Other than GFAP accumulation, astrogliosis is also characterized by astrocyte proliferation (hyperplasia) and extensive hypertrophy of the cell body, nucleus as well as cytoplasmic processes (Eng, 1988a). Astrogliosis may participate in the healing phase following CNS injury by actively monitoring and controlling the molecular and ionic contents of the extracellular space of the CNS. They can wall off areas of the CNS that are exposed to non-CNS tissue environments following trauma. On the other hand, such responses may interfere with the function of residual neuronal circuits, by preventing remyelination, or by inhibiting axonal regeneration (Eng et al., 1987; Stensaas et al., 1987; Reier and Houle, 1988). Although astrogliosis has received considerable attention in term of its proposed inhibitory effect on CNS repair, there is still very little specific information available concerning the properties of reactive astrocytes, what triggers glial reactivity, and many of the cellular dynamics associated with scar formation. Control of astrocyte proliferation, differentiation, and astrogliosis may be linked to GFAP synthesis. Our aim was to transfect astrocytes with exogenous synthetic oligo- or polynucleotides, which would allow the manipulation of a transient suppression of GFAP synthesis which might delay the gliotic reaction and the scar formation, thus allowing neurons and oligodendrocytes to re-establish a functional environment.

Keywords

Glial Fibrillary Acidic Protein Astrocyte Proliferation Antisense Construct Antisense mRNA Glial Fibrillary Acidic Protein mRNA 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1993

Authors and Affiliations

  • Albert C. H. Yu
    • 1
  • Yuen Ling Lee
    • 1
  • Lawrence F. Eng
    • 2
  1. 1.Department of Pathology, School of MedicineStanford UniversityStanfordUSA
  2. 2.VA Medical CenterPalo AltoUSA

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