Identification of Hydrogen Peroxide as the Relevant Messenger in the Activation Pathway of Transcription Factor NF-κB

  • Kerstin N. Schmidt
  • Paul Amstad
  • Peter Cerutti
  • Patrick A. Baeuerle
Part of the Advances in Experimental Medicine and Biology book series (AEMB, volume 387)

Abstract

The inducible higher eukaryotic transcription factor NF-κB is activated by a large variety of distinct simuli [1–3]. In unstimulated cells, this factor resides in a latent form in the cytoplasm [4]. Latency is achieved by association of the DNA-binding NF-κB dimer with an inhibitory subunit, called IκB [5]. IΚB suppresses DNA-binding and nuclear transport of NF-κB. Upon stimulation of cells, IκB is phosphorylated and proteolytically degraded [6–9]. Both reactions are required for activation [10]. The released NF-κB is then translocated to the nucleus where it initiates transcription of target genes. Among the numerous proteins which are induced by a concerted action of NF-kB with other transcription factors are cytokines, chemokines, cell adhesion molecules, hematopoetic growth factors and receptors, histocompatibility antigens and acute phase proteins [1–3]. While NF-κB may be indispensable as inducer of many immediate-early inflammatory and immune reactions, the transcription factor is likely to play a fatal role in certain diseases and syndromes that involve an abberrant expression of inflammatory cytokines [22–24].

Keywords

Tumor Necrosis Factor Electrophoretic Mobility Shift Assay Okadaic Acid Total Cell Extract Inhibitory Subunit 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer Science+Business Media New York 1996

Authors and Affiliations

  • Kerstin N. Schmidt
    • 1
  • Paul Amstad
    • 2
  • Peter Cerutti
    • 2
  • Patrick A. Baeuerle
    • 1
  1. 1.Institute of BiochemistryUniversity of FreiburgFreiburgGermany
  2. 2.Department of CarcinogenesisSwiss Institute for Experimental Cancer ResearchÉpalinges s. LausanneSwitzerland

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