Urinanalysis by 1H NMR: Application to Cephaloridine-Treated Rats

  • R. Pickford
  • I. K. Smith
  • I. D. Wilson
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)

Abstract

Although a quite recent development the analysis of biological fluids by high-resolution proton NMR is rapidly becoming an established procedure, especially for urine and plasma (e.g. [1–3], and arts, by J.K. Nicholson & colleagues in this book and in Vols. 16 & 17). A particular advantage is the wealth of additional information furnished, over and above that required for the qualitative or quantitative determination of a single analyte that is manifest in the spectrum from the sample.- Any organic molecule possessing protons, will be detected, provided that the levels are mM or higher. Accordingly, a single assay, on otherwise untreated urine samples, can comprehend drugs, drug metabolites, and endogenous metabolites (e.g. Krebs cycle intermediates, sugars, organic acids, creatinine and various amino acids) that may bear on drug-related toxicicity. No Chromatographic method could be as simple and wide-ranging as 1H NMR. These points are now exemplified by 1H NMR observations on urine of rats dosed with a nephrotoxic cephalosporin antibiotic, cephaloridine.

Keywords

Hippuric Acid Endogenous Metabolite Single Analyte Krebs Cycle Intermediate Field Frequency Lock 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Wilson, I.D., Fromson, J., Ismail, I.M. & Nicholson, J.K. (1987) J. Pharm. Biomed. Anal. 5, 157–163.CrossRefGoogle Scholar
  2. 2.
    Nicholson, J.K. & Wilson, I.D. (1987) in Drug Metabolism — from Molecules to Man (Benford, D.J., Bridges, J.W. & Gibson, G.G., eds.), Taylor & Francis, London, pp. 189–207.Google Scholar
  3. 3.
    Nicholson, J.K. & Wilson, I.D. (1987) Progr. Drug Res. 31 [Jucker, E., ed.], 427–479.Google Scholar
  4. 4.
    Connor, S., Everett, J. & Nicholson, J.K. (1987) Mag. Res. Med. 4, 461–470.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • R. Pickford
    • 1
  • I. K. Smith
    • 1
  • I. D. Wilson
    • 1
  1. 1.ICI Pharmaceuticals Division MeresideMacclesfield, CheshireUK

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