The Use of Fluorescence and Chemiluminescence Techniques for Sensitive and Selective Detection in HPLC

  • U. A. Th. Brinkman
  • G. J. de Jong
  • C. Gooijer
Part of the Methodological Surveys in Biochemistry and Analysis book series (MSBA, volume 18 A)

Abstract

Luminescence techniques, including CL*, can be especially useful for HPLC detection when high sensitivity and/or selectivity are required, and they can be nicely adapted to the demands of miniaturized HPLC. In this article four aspects are discussed: (i) on-line post-column reaction detection in HPLC with conventional fluorescence monitoring; (ii) the state-of-the-art of laser-induced fluorescence detection for HPLC; (iii) recent progress in multidimensional fluorescence detection; and (iv) the principles and application of on-line CL (peroxyoxalate system) detection in HPLC.

Keywords

Reaction Coil HPLC Detection HPLC Eluent Microbore Column Dansylated Amino Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviation

CL

chemiluminescence

OPA

0-phthalaldehyde

DAS

9,10-dimethoxyanthracene-2-sulphonate

LIF

laser-induced fluorescence

OTLC

open-tubular HPLC

EEM

emissionexcitation matrix

ILDA

intensified linear photodiode array

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Copyright information

© Springer Science+Business Media New York 1988

Authors and Affiliations

  • U. A. Th. Brinkman
    • 1
  • G. J. de Jong
    • 1
  • C. Gooijer
    • 1
  1. 1.Department of Analytical ChemistryFree UniversityAmsterdamThe Netherlands

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